Overview

  • Product name
    Anti-Saporin antibody (HRP)
    See all Saporin primary antibodies
  • Description
    Goat polyclonal to Saporin (HRP)
  • Host species
    Goat
  • Conjugation
    HRP
  • Tested applications
    Suitable for: SDS-PAGEmore details
  • Species reactivity

    Predicted to work with: Soapwort
  • Immunogen

    Full length protein corresponding to Saporin. Soapwort plant (Saponaria officinalis).

  • General notes

    The antibody was coupled to Horseradish Peroxidase (HRP) and dialysed against PBS.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Constituents: PBS, 0.81% Sodium chloride, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.16% Sodium phosphate
  • Concentration information loading...
  • Purification notes
    The antibody was conjugated to horseradish peroxidase (HRP) and dialyzed against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use a concentration of 1 - 10 µg/ml.

Target

  • Relevance
    Saporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha 2 macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death. Among all type I RIPs saporin SO6 is the most widely used because it is easily obtainable and very stable.
  • Database links
  • Alternative names
    • Saporin antibody

Images

  • Saporin was run on a 10% SDS-PAGE gel and transferred to a PVDF membrane. The blot was blocked with 4% NFM/TBS, then incubated overnight with 5 µg/ml ab42903. The blot was washed and developed with 4-chloro-1-naphthol and hydrogen peroxide.

    Lane 1: 200 ng saporin.

    Lane 2: 100 ng saporin.

    Lane 3: 50 ng saporin.

    Lane 4: 25 ng saporin.

     

References

ab42903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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