Product nameAnti-Saporin antibody (HRP)
See all Saporin primary antibodies
DescriptionGoat polyclonal to Saporin (HRP)
Tested applicationsSuitable for: SDS-PAGEmore details
Predicted to work with: Soapwort
Full length protein corresponding to Saporin. Soapwort plant (Saponaria officinalis).
The antibody was coupled to Horseradish Peroxidase (HRP) and dialysed against PBS.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Constituents: PBS, 0.81% Sodium chloride, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.16% Sodium phosphate
Concentration information loading...
Purification notesThe antibody was conjugated to horseradish peroxidase (HRP) and dialyzed against PBS.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab42903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||Use a concentration of 1 - 10 µg/ml.|
RelevanceSaporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha 2 macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death. Among all type I RIPs saporin SO6 is the most widely used because it is easily obtainable and very stable.
- SwissProt: P20656 Soapwort
- Saporin antibody
Saporin was run on a 10% SDS-PAGE gel and transferred to a PVDF membrane. The blot was blocked with 4% NFM/TBS, then incubated overnight with 5 µg/ml ab42903. The blot was washed and developed with 4-chloro-1-naphthol and hydrogen peroxide.
Lane 1: 200 ng saporin.
Lane 2: 100 ng saporin.
Lane 3: 50 ng saporin.
Lane 4: 25 ng saporin.
ab42903 has not yet been referenced specifically in any publications.