Overview

  • Product name

    Anti-SAPS1 antibody
  • Description

    Rabbit polyclonal to SAPS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 841 and the C-terminus (residue 891) of human SAPS1, NP_055746.2.

  • Positive control

    • Whole cell lysate from Hela cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 103 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance

    Protein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit. It is ubiquitous, with higher expression in testis. There are two named isoforms.
  • Cellular localization

    Cytoplasm
  • Database links

  • Alternative names

    • KIAA1115 antibody
    • PP6R1 antibody
    • Protein phosphatase 6, regulatory subunit 1 antibody
    • SAP190 antibody
    • SAPS domain family, member 1 antibody
    see all

Images

  • All lanes : Anti-SAPS1 antibody (ab72030) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg

    Predicted band size: 103 kDa
    Observed band size: 117 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation/ Western Blot of SAPS1.
    Lane 1: ab72030 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    ab72030 at 0.1 µg/ml was used for the western blot detection step. Chemiluminescence with an exposure time of 3 seconds.

References

ab72030 has not yet been referenced specifically in any publications.

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