Key features and details
- Rabbit polyclonal to SAPS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAPS1 antibody
DescriptionRabbit polyclonal to SAPS1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide from a region between residue 841 and the C-terminus (residue 891) of human SAPS1, NP_055746.2.
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 103 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceProtein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit. It is ubiquitous, with higher expression in testis. There are two named isoforms.
- KIAA1115 antibody
- PP6R1 antibody
- Protein phosphatase 6, regulatory subunit 1 antibody
All lanes : Anti-SAPS1 antibody (ab72030) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 103 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Immunoprecipitation/ Western Blot of SAPS1.
Lane 1: ab72030 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. ab72030 at 0.1 µg/ml was used for the western blot detection step. Chemiluminescence with an exposure time of 3 seconds.
ab72030 has not yet been referenced specifically in any publications.