Key features and details
- Rabbit polyclonal to SAPS2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAPS2 antibody
See all SAPS2 primary antibodies
DescriptionRabbit polyclonal to SAPS2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide from a region between residue 775 and 825 of human SAPS2, NP_055493.2.
- Whole cell lysate from Hela and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects bands of approximately 130 and 150 kDa (predicted molecular weight: 105 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceSAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C). Protein phosphatase regulatory subunits, such as SAPS2, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. It is ubiquitously expressed with strongest expression in the testis followed by liver, heart, kidney, brain and placenta. There are two named isoforms.
- SAPS 2 antibody
- dJ579N16.1 antibody
- KIAA0685 antibody
All lanes : Anti-SAPS2 antibody (ab72031) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of SAPS2.
Lane 1: ab72031 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. ab72031 at 1 µg/ml was used for the Western blot detection step.
Chemiluminescence with an exposure time of 3 seconds.
ab72031 has not yet been referenced specifically in any publications.