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Rabbit polyclonal to SAPS3
Predicted to work with:
Synthetic peptide from a region between residue 743 and the C-terminus
(residue 793) of human SAPS3,( NP_060782.2).
Whole cell lysate from Hela cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
Use at 2-5 µg/mg of lysate.
Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance.
Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes.
Belongs to the SAPS family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Chromosome 11 open reading frame 23 antibody
Western blot - Anti-SAPS3 antibody (ab72034)
All lanes :
Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml
Lane 1 :
Whole cell lysate from Hela cells at 50 µg
Lane 2 :
Whole cell lysate from Hela cells at 15 µg
Lane 3 :
Whole cell lysate from Hela cells at 5 µg
Lane 4 :
Whole cell lysate from 293T cells at 50 µg
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-SAPS3 antibody (ab72034)
Immunoprecipitation/ Western Blot of SAPS3.
Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
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