Recombinant
RabMAb

Recombinant Anti-SAR1B antibody [EPR10105(B)] (ab155278)

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Overview

  • Product name

    Anti-SAR1B antibody [EPR10105(B)]
    See all SAR1B primary antibodies

  • Description

    Rabbit monoclonal [EPR10105(B)] to SAR1B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human SAR1B (UniProt ID: Q9Y6B6).

  • Positive control

    • 293T, fetal liver, fetal brain and Human skeletal muscle lysates; Human gastric carcinoma and brain tissue; 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab155278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.

  • Tissue specificity

    Expressed in many tissues including small intestine, liver, muscle and brain.

  • Involvement in disease

    Defects in SAR1B are the cause of chylomicron retention disease (CMRD) [MIM:246700]; also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.

  • Sequence similarities

    Belongs to the small GTPase superfamily. SAR1 family.

  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ANDD antibody
    • CMRD antibody
    • GTBPB antibody
    • GTP binding protein SAR1b antibody
    • GTP binding protein Sara antibody
    • GTP-binding protein B antibody
    • GTP-binding protein SAR1B antibody
    • SAR1 gene homolog B (S. cerevisiae) antibody
    • SAR1 homolog B antibody
    • SAR1a gene homolog 2 antibody
    • SAR1B antibody
    • SAR1B_HUMAN antibody
    • SARA2 antibody
    • SARB antibody
    see all

Images

  • Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    All lanes : Anti-SAR1B antibody [EPR10105(B)] (ab155278) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAR1B with ab155278 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)

    Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling SAR1B with ab155278 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Immunocytochemistry/ Immunofluorescence - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Immunofluorescence analysis of 293T cells labeling SAR1B with ab155278 at a 1/50 dilution.
  • Flow Cytometry - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Flow Cytometry - Anti-SAR1B antibody [EPR10105(B)] (ab155278)
    Flow cytometric analysis of permeabilized 293T cells using ab155278 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

References

Publishing research using ab155278? Please let us know so that we can cite the reference in this datasheet.

ab155278 has been referenced in 1 publication.

  • Smagris E  et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 291:10659-76 (2016). WB . PubMed: 27013658

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SAR1B antibody [EPR10105(B)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

Submitted Mar 14 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-SAR1B antibody [EPR10105(B)]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

Submitted Aug 18 2015

Western blot abreview for Anti-SAR1B antibody [EPR10105(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
35 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Armen Petrosyan

Verified customer

Submitted Jul 31 2015

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