Recombinant Anti-SAR1B antibody [EPR10105(B)] (ab155278)
![Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/ab155278-162353-anti-sar1b-antibody-epr10105b-western-blot.jpg "Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10105(B)] to SAR1B
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SAR1B antibody [EPR10105(B)]
See all SAR1B primary antibodies -
Description
Rabbit monoclonal [EPR10105(B)] to SAR1B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to residues in Human SAR1B (UniProt ID: Q9Y6B6).
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Positive control
- 293T, fetal liver, fetal brain and Human skeletal muscle lysates; Human gastric carcinoma and brain tissue; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10105(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
1/50 - 1/100.
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/50 - 1/100. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. -
Tissue specificity
Expressed in many tissues including small intestine, liver, muscle and brain. -
Involvement in disease
Defects in SAR1B are the cause of chylomicron retention disease (CMRD) [MIM:246700]; also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. -
Sequence similarities
Belongs to the small GTPase superfamily. SAR1 family. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region. - Information by UniProt
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Database links
- Entrez Gene: 51128 Human
- Entrez Gene: 66397 Mouse
- Entrez Gene: 287276 Rat
- Omim: 607690 Human
- SwissProt: Q9Y6B6 Human
- SwissProt: Q9CQC9 Mouse
- SwissProt: Q5HZY2 Rat
- Unigene: 432984 Human
see all -
Alternative names
- ANDD antibody
- CMRD antibody
- GTBPB antibody
see all
Images
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Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)All lanes : Anti-SAR1B antibody [EPR10105(B)] (ab155278) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : Fetal liver lysate
Lane 3 : Fetal brain lysate
Lane 4 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/ab155278-162352-anti-sar1b-antibody-epr10105b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAR1B with ab155278 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/ab155278-162351-anti-sar1b-antibody-epr10105b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling SAR1B with ab155278 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/ab155278-162350-anti-sar1b-antibody-epr10105b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Immunofluorescence analysis of 293T cells labeling SAR1B with ab155278 at a 1/50 dilution. -
![Flow Cytometry - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/ab155278-162349-anti-sar1b-antibody-epr10105b-flow-cytometry.jpg)
Flow Cytometry - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Flow cytometric analysis of permeabilized 293T cells using ab155278 at a 1/10 dilution (red) or a rabbit IgG (negative) (green). -
![Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/ab155278-6-benefits-of-recombinant-antibodies.png)
Anti-SAR1B antibody [EPR10105(B)] (ab155278)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155278 has been referenced in 3 publications.
- Lu Y et al. Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells. Oncol Lett 20:186 (2020). PubMed: 32952655
- Frisbie CP et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin. Cells 8:N/A (2019). PubMed: 31847122
- Smagris E et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 291:10659-76 (2016). WB . PubMed: 27013658