Recombinant
Anti-SAR1B antibody [EPR10105(B)] (ab155278)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-SAR1B antibody [EPR10105(B)]
-
DescriptionRabbit monoclonal [EPR10105(B)] to SAR1B
-
Host speciesRabbit
-
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide corresponding to residues in Human SAR1B (UniProt ID: Q9Y6B6).
-
Positive control
- 293T, fetal liver, fetal brain and Human skeletal muscle lysates; Human gastric carcinoma and brain tissue; 293T cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20ºC.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR10105(B)
-
IsotypeIgG
-
Research areas
Associated products
-
Isotype control
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab155278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 22 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionInvolved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.
-
Tissue specificityExpressed in many tissues including small intestine, liver, muscle and brain.
-
Involvement in diseaseDefects in SAR1B are the cause of chylomicron retention disease (CMRD) [MIM:246700]; also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.
-
Sequence similaritiesBelongs to the small GTPase superfamily. SAR1 family.
-
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region.
- Information by UniProt
-
Database links
- Entrez Gene: 51128 Human
- Entrez Gene: 66397 Mouse
- Entrez Gene: 287276 Rat
- Omim: 607690 Human
- SwissProt: Q9Y6B6 Human
- SwissProt: Q9CQC9 Mouse
- SwissProt: Q5HZY2 Rat
- Unigene: 432984 Human
see all -
Alternative names
- ANDD antibody
- CMRD antibody
- GTBPB antibody
see all
Images
-
![Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/SAR1B-Primary-antibodies-ab155278-1.jpg)
Western blot - Anti-SAR1B antibody [EPR10105(B)] (ab155278)All lanes : Anti-SAR1B antibody [EPR10105(B)] (ab155278) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : Fetal liver lysate
Lane 3 : Fetal brain lysate
Lane 4 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/SAR1B-Primary-antibodies-ab155278-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAR1B with ab155278 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/SAR1B-Primary-antibodies-ab155278-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling SAR1B with ab155278 at 1/50 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/SAR1B-Primary-antibodies-ab155278-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Immunofluorescence analysis of 293T cells labeling SAR1B with ab155278 at a 1/50 dilution. -
![Flow Cytometry - Anti-SAR1B antibody [EPR10105(B)] (ab155278)](https://www.abcam.com/ps/products/155/ab155278/Images/SAR1B-Primary-antibodies-ab155278-5.jpg)
Flow Cytometry - Anti-SAR1B antibody [EPR10105(B)] (ab155278)Flow cytometric analysis of permeabilized 293T cells using ab155278 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
Protocols
References
This product has been referenced in:
- Smagris E et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 291:10659-76 (2016). WB . Read more (PubMed: 27013658) »
