Overview

  • Product name
    Anti-SAR1B antibody [EPR10105(B)]
  • Description
    Rabbit monoclonal [EPR10105(B)] to SAR1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SAR1B (UniProt ID: Q9Y6B6).

  • Positive control
    • 293T, fetal liver, fetal brain and Human skeletal muscle lysates; Human gastric carcinoma and brain tissue; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.
  • Tissue specificity
    Expressed in many tissues including small intestine, liver, muscle and brain.
  • Involvement in disease
    Defects in SAR1B are the cause of chylomicron retention disease (CMRD) [MIM:246700]; also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.
  • Sequence similarities
    Belongs to the small GTPase superfamily. SAR1 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANDD antibody
    • CMRD antibody
    • GTBPB antibody
    • GTP binding protein SAR1b antibody
    • GTP binding protein Sara antibody
    • GTP-binding protein B antibody
    • GTP-binding protein SAR1B antibody
    • SAR1 gene homolog B (S. cerevisiae) antibody
    • SAR1 homolog B antibody
    • SAR1a gene homolog 2 antibody
    • SAR1B antibody
    • SAR1B_HUMAN antibody
    • SARA2 antibody
    • SARB antibody
    see all

Images

  • All lanes : Anti-SAR1B antibody [EPR10105(B)] (ab155278) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa

  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAR1B with ab155278 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling SAR1B with ab155278 at 1/50 dilution.
  • Immunofluorescence analysis of 293T cells labeling SAR1B with ab155278 at a 1/50 dilution.
  • Flow cytometric analysis of permeabilized 293T cells using ab155278 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

References

This product has been referenced in:
  • Smagris E  et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 291:10659-76 (2016). WB . Read more (PubMed: 27013658) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Mar 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Aug 18 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
35 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Jul 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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