Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionEarly endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
Tissue specificityUbiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
Sequence similaritiesContains 1 FYVE-type zinc finger.
DomainThe SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3.
The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- CG15667 antibody
- hSARA antibody
- LD33044p antibody
ab221488 has not yet been referenced specifically in any publications.