• Product name

    Anti-SARA antibody [EPR5020]
    See all SARA primary antibodies
  • Description

    Rabbit monoclonal [EPR5020] to SARA
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human SARA aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Huan heart, A673, HT-1080, BxPC-3, Jutkat, HeLa, and Human fetal brain lysates, Human kidney tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
    • Tissue specificity

      Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
    • Sequence similarities

      Contains 1 FYVE-type zinc finger.
    • Domain

      The SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3.
      The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
    • Cellular localization

      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CG15667 antibody
      • hSARA antibody
      • LD33044p antibody
      • Madh-interacting protein antibody
      • MADHIP antibody
      • Mothers against decapentaplegic homolog-interacting protein antibody
      • Novel serine protease antibody
      • NSP antibody
      • Receptor activation anchor antibody
      • SAR1 antibody
      • SARA antibody
      • SARA1 antibody
      • Smad anchor for receptor activation antibody
      • SMADIP antibody
      • ZFYV9_HUMAN antibody
      • ZFYVE9 antibody
      • Zinc finger FYVE domain-containing protein 9 antibody
      see all


    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HT-1080 cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 156 kDa

    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 156 kDa

    • Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:

    • Jing C  et al. MicroRNA-17/20a impedes migration and invasion via TGF-ß/ITGB6 pathway in esophageal squamous cell carcinoma. Am J Cancer Res 6:1549-62 (2016). Read more (PubMed: 27508097) »
    • Correia AC  et al. FGF2 inhibits endothelial-mesenchymal transition through microRNA-20a-mediated repression of canonical TGF-ß signaling. J Cell Sci 129:569-79 (2016). Read more (PubMed: 26729221) »
    See all 3 Publications for this product

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