Anti-Sarcalumenin antibody [XIIC4] (ab2874)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Sarcalumenin antibody [XIIC4]
See all Sarcalumenin primary antibodies -
Description
Mouse monoclonal [XIIC4] to Sarcalumenin -
Host species
Mouse -
Specificity
This antibody specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant. -
Tested applications
Suitable for: ICC/IF, WB, Inhibition Assay, IP, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Guinea pig, Dog, Human -
Immunogen
Full length native protein (purified) corresponding to Rabbit Sarcalumenin. Purified from rabbit sarcoplasmic reticulum membrane vesicles.
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Positive control
- Rabbit skeletal muscle extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner.The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration. -
Clonality
Monoclonal -
Clone number
XIIC4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/500. Immunofluorescence staining of sarcalumenin and its splice variant in rabbit skeletal muscle results in staining that co-localizes with calcium ATPase in the longitudinal sarcoplasmic reticulum (SR) and the terminal cisternae. |
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WB | 1/500. By Western blot, this antibody detects a 53 kDa and a 160 kDa protein representing the glycoprotein and sarcalumenin, respectively, in rabbit skeletal muscle extracts. |
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Inhibition Assay | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. | |
IHC-Fr | 1/100. |
Target
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Function
May be involved in the regulation of calcium transport. -
Cellular localization
Sarcoplasmic reticulum lumen. Associated through calcium with the membrane. - Information by UniProt
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Database links
- Entrez Gene: 6345 Human
- Entrez Gene: 106393 Mouse
- Omim: 604992 Human
- SwissProt: Q86TD4 Human
- SwissProt: Q7TQ48 Mouse
- SwissProt: P13666 Rabbit
- Unigene: 10041 Human
- Unigene: 35811 Mouse
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Alternative names
- Sarcalumenin antibody
- SRCA_HUMAN antibody
- SRL antibody
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kutchai H & Campbell KP Calcium transport by sarcoplasmic reticulum of skeletal muscle is inhibited by antibodies against the 53-kilodalton glycoprotein of the sarcoplasmic reticulum membrane. Biochemistry 28:4830-9 (1989). Read more (PubMed: 2527558) »