Product nameAnti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies
DescriptionRabbit polyclonal to Sarcomeric Alpha Actinin
Tested applicationsSuitable for: WB, IHC-P, ICC, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Monkey, Zebrafish
Recombinant Human Sarcomeric Alpha Actinin.
- Human Heart and Skeletal Muscle tissues; A431 and H1299 whole cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab113086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 104 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/200.|
FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificityExpressed in both skeletal and cardiac muscle.
Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
- Information by UniProt
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry.
ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Skeletal muscle tissue by Immunohistochemistry.
All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab113086) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
7.5% SDS PAGE.
ab112086 at 1/200 dilution staining Sarcomeric Alpha Actinin in Paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
ab113086 has not yet been referenced specifically in any publications.