Anti-Sarcomeric Alpha Actinin antibody (ab113086)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Monkey, Zebrafish -
Immunogen
Recombinant Human Sarcomeric Alpha Actinin.
-
Positive control
- Human Heart and Skeletal Muscle tissues; A431 and H1299 whole cell lysates; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
|
|
IHC-P |
Use a concentration of 10 µg/ml.
|
|
ICC/IF |
1/100 - 1/200.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
1/100 - 1/200. |
Target
-
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
-
Database links
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Entrez Gene: 641575 Zebrafish
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
see all -
Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
-
ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry.
-
ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Skeletal muscle tissue by Immunohistochemistry.
-
All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab113086) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
7.5% SDS PAGE. -
ab112086 at 1/200 dilution staining Sarcomeric Alpha Actinin in Paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab113086 has not yet been referenced specifically in any publications.