Anti-Sarcomeric Alpha Actinin antibody (ab137346)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human Sarcomeric Alpha Actinin. The immunogen is within the center region of the protein.
Database link: P35609 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 103 kDa.
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ICC/IF | (4) |
1/100 - 1/1000.
Use ice-cold Methanol as fixation. |
IHC-P |
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 103 kDa. |
ICC/IF
1/100 - 1/1000. Use ice-cold Methanol as fixation. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 396263 Chicken
- Entrez Gene: 536607 Cow
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Entrez Gene: 432169 Xenopus laevis
- Entrez Gene: 448602 Xenopus tropicalis
- Omim: 102573 Human
see all -
Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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Immunofluorescent analysis of ice-cold methanol fixed (5 minutes) HeLa cells labeling Sarcomeric Alpha Actinin with ab137346 at 1/500 dilution (4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) (keep from light) at 1/2000 dilution (room temperature for 1 hour) (green). Blue: Hoechst 33342 staining.
Blocking: 2.5% BSA/PBS for 30 minutes.
Washing: PBS, 3 x 3 minutes.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab137346) at 1/1000 dilution
Lane 1 : 293T cells
Lane 2 : A431 cells
Lane 3 : HeLa cells
Lane 4 : HepG2 cells
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated anti-rabbit IgG antibody
Predicted band size: 103 kDa5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 10 µg/ml. Antigen retrieval: EDTA based, pH 8.0, buffer, 15minutes.
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 1/2000. Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab137346) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate/extract
Lane 2 : GL261 whole cell lysate/extract
Lane 3 : C8D30 whole cell lysate/extract
Lane 4 : NIH-3T3 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 103 kDa -
Immunohistochemical analysis of paraffin-embedded rat heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 1/2000. Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab137346 has been referenced in 35 publications.
- Liu Y et al. hESCs-Derived Early Vascular Cell Spheroids for Cardiac Tissue Vascular Engineering and Myocardial Infarction Treatment. Adv Sci (Weinh) 9:e2104299 (2022). PubMed: 35092352
- Wang Y et al. A rare case of arrhythmogenic right ventricular cardiomyopathy associated with LAMA2 mutation: A case report and literature review. Front Med (Lausanne) 9:922347 (2022). PubMed: 35924034
- Jayne RK et al. Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers. Lab Chip 21:1724-1737 (2021). PubMed: 33949395
- Abdel-Raouf KMA et al. Transdifferentiation of Human Fibroblasts into Skeletal Muscle Cells: Optimization and Assembly into Engineered Tissue Constructs through Biological Ligands. Biology (Basel) 10:N/A (2021). PubMed: 34208436
- Brandt EB et al. Activation of P53 Via Nutlin-3a Reveals Role for P53 In ROS Signaling During Cardiac Differentiation of hiPSCs. J Stem Cell Rep 3:N/A (2021). PubMed: 34485982