Anti-Sarcomeric Alpha Actinin antibody (ab227216)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig -
Immunogen
Recombinant fragment within Human Sarcomeric Alpha Actinin (internal sequence). The exact sequence is proprietary.
Database link: P35609 -
Positive control
- WB: A431, NCI-H1299 and NIH/3T3 whole cell lysates; Rat heart tissue extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/10000. Predicted molecular weight: 104 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 104 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 396263 Chicken
- Entrez Gene: 536607 Cow
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: Q3ZC55 Cow
- SwissProt: P35609 Human
see all -
Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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Paraffin-embedded human breast carcinoma tissue stained for Sarcomeric Alpha Actinin using ab227216 at 1/250 dilution in immunohistochemical analysis.
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Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/3000 dilution + NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 104 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa7.5% SDS-PAGE gel.
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Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/5000 dilution + Rat heart tissue extract at 50 µg
Predicted band size: 104 kDa7.5% SDS-PAGE gel.
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PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Sarcomeric Alpha Actinin (green) using ab227216 at 1/200 dilution in ICC/IF.
Right-hand panel: Merged with DNA probe.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227216 has been referenced in 1 publication.
- Altemus J et al. Adipose tissue-derived mesenchymal stem cells' acellular product extracellular vesicles as a potential therapy for Crohn's disease. J Cell Physiol 237:3001-3011 (2022). PubMed: 35522572