Overview

  • Product name

    Anti-Sarcomeric Alpha Actinin antibody
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description

    Rabbit polyclonal to Sarcomeric Alpha Actinin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig
  • Immunogen

    Recombinant fragment within Human Sarcomeric Alpha Actinin (internal sequence). The exact sequence is proprietary.
    Database link: P35609

  • Positive control

    • WB: A431, NCI-H1299 and NIH/3T3 whole cell lysates; Rat heart tissue extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 104 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificity

    Expressed in both skeletal and cardiac muscle.
  • Involvement in disease

    Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities

    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localization

    Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin binding protein antibody
    • Actinin alpha 2 antibody
    • ACTN 2 antibody
    • ACTN2 antibody
    • ACTN2_HUMAN antibody
    • Alpha actinin 2 antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin-2 antibody
    • CMD1AA antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    see all

Images

  • Paraffin-embedded human breast carcinoma tissue stained for Sarcomeric Alpha Actinin using ab227216 at 1/250 dilution in immunohistochemical analysis.

  • Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/3000 dilution + NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 104 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 104 kDa



    7.5% SDS-PAGE gel.

  • Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/5000 dilution + Rat heart tissue extract at 50 µg

    Predicted band size: 104 kDa



    7.5% SDS-PAGE gel.

  • PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Sarcomeric Alpha Actinin (green) using ab227216 at 1/200 dilution in ICC/IF.

    Right-hand panel: Merged with DNA probe.

References

ab227216 has not yet been referenced specifically in any publications.

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