Product nameAnti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies
DescriptionRabbit polyclonal to Sarcomeric Alpha Actinin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow
Synthetic peptide corresponding to Human Sarcomeric Alpha Actinin aa 850 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following human tissue lysates: Skeletal Muscle; Heart.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab90776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).|
FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificityExpressed in both skeletal and cardiac muscle.
Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
- Information by UniProt
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab90776) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 27 kDa, 30 kDa, 45 kDa, 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
IHC image of Sarcomeric Alpha Actinin staining in human Heart FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90776, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
This product has been referenced in:
- Huang Y et al. Age-Dependent Oxidative DNA Damage Does Not Correlate with Reduced Proliferation of Cardiomyocytes in Humans. PLoS One 12:e0170351 (2017). Read more (PubMed: 28099512) »
- Ma ZG et al. Piperine Attenuates Pathological Cardiac Fibrosis Via PPAR-?/AKT Pathways. EBioMedicine 18:179-187 (2017). Read more (PubMed: 28330809) »