Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (ab257317)
Key features and details
- Mouse monoclonal [ACTN2/3292] to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB, Protein Array
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292]
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Mouse monoclonal [ACTN2/3292] to Sarcomeric Alpha Actinin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Sarcomeric Alpha Actinin aa 557-692. The exact sequence is proprietary.
Database link: P35609 -
Positive control
- IHC-P: Human cardiac muscle and skeletal muscle tissue. WB: Human skeletal muscle tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
ACTN2/3292 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab257317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature. |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 103 kDa.
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Protein Array |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 103 kDa. |
Protein Array
Use at an assay dependent concentration. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 88 Human
- Omim: 102573 Human
- SwissProt: P35609 Human
- Unigene: 498178 Human
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Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human cardiac muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (ab257317) at 2 µg/ml + Human skeletal muscle tissue lysate
Predicted band size: 103 kDa -
Analysis of >19,00 full-length human proteins using ab257317.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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Formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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Formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab257317 has not yet been referenced specifically in any publications.