Product nameAnti-Sarcomeric Alpha Actinin antibody [EA-53]
See all Sarcomeric Alpha Actinin primary antibodies
DescriptionMouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
SpecificityAccording to the following publication, this antibody clone detects the Alpha Actinin 2. However, we have not confirmed this with internal testing if it detects Actinin 1 or 2. https://academic.oup.com/hmg/article/14/12/1587/640357/Alpha-actinin-associates-with-polycystin-2-and
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, WBmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Dog, Human, Pig, Xenopus laevis, Fish, Lizard, Snake, Xenopus tropicalis
Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
- WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17383364|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20019242|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).|
FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificityExpressed in both skeletal and cardiac muscle.
Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
- Information by UniProt
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
ab9465 staining Sarcomeric Alpha Actinin in mouse heart tissue by Immunohistochemistry (Frozen sections). Serial longitudinal (12 µm) sections from wt hearts were incubated with ab9465. Nuclei were visualized with DAPI.
Immunocytochemistry/Immunofluorescence analysis of H9 hESC and CBiPSC6.2 cells labelling Sarcomeric Alpha Actinin (green) with ab9465 at a dilution of 1/200. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/200).
Immunofluorescence staining of SNAIL1 and cardiac cell type markers (alpha actinin-cardiomyocyte marker; 1/100 dilution) in the ventricular infarct zone of LAD surgery mice. Scale bars: 50 microns.
5 um sections of paraffin or OCT embedded hearts were utilized for Massons Trichrome Staining and immunofluorescence. For SNAIL1 immunofluorescence, antigen retrieval was done in Nuclear Decloaker solution and TSA plus kit was used to amplify SNAIL1 signal.
All lanes : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/250 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lane 3 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 103 kDa
Additional bands at: 25 kDa, 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue staining Sarcomeric Alpha Actinin with ab9465.
This product has been referenced in:
- Fu H et al. Dioxin and AHR impairs mesoderm gene expression and cardiac differentiation in human embryonic stem cells. Sci Total Environ 651:1038-1046 (2019). Read more (PubMed: 30266049) »
- Redd MA et al. Patterned human microvascular grafts enable rapid vascularization and increase perfusion in infarcted rat hearts. Nat Commun 10:584 (2019). Read more (PubMed: 30718840) »