Recombinant Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
- Datasheet
- References (23)
- Protocols
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2529Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab68167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). | |
| IP | 1/20 - 1/40. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
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Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-4-ab68167IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-8-ab68167WB3.jpg)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167ihc2 copy.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-3-ab68167IP1.jpg)
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab68167 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes :
Lane 1 : HepG2 whole cell lysate at 10 µg
Lane 2 : ab68167 + HepG2 whole cell lysate at 10 µg
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate
Observed band size: 103 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-5-ab68167IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167wb.gif)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat heart lysate
Lane 3 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa -
![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-7-ab68167WB2.jpg)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-6-ab68167WB1.jpg)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
Datasheets and documents
References
This product has been referenced in:
- Lornage X et al. ACTN2 mutations cause "Multiple structured Core Disease" (MsCD). Acta Neuropathol 137:501-519 (2019). Read more (PubMed: 30701273) »
- Hong SP et al. Regenerative potential of mouse embryonic stem cell-derived PDGFRa+ cardiac lineage committed cells in infarcted myocardium. World J Stem Cells 11:44-54 (2019). Read more (PubMed: 30705714) »
