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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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RecombinantRabMAb

Recombinant Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (29)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
  • Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description

    Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2529Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

Associated products

  • Alternative Versions

    • Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse heart tissue lysate - total protein (0 days) (ab7193)
    • Mouse heart tissue lysate - total protein (14 days) (ab7194)
    • Mouse heart tissue lysate - total protein (7 days) (ab7196)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Human
IP
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IP
1/20 - 1/40.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IP
1/20 - 1/40.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Tissue specificity

      Expressed in both skeletal and cardiac muscle.
    • Involvement in disease

      Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localization

      Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
    • Target information above from: UniProt accession P35609 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 88 Human
      • Entrez Gene: 11472 Mouse
      • Entrez Gene: 291245 Rat
      • Omim: 102573 Human
      • SwissProt: P35609 Human
      • SwissProt: Q9JI91 Mouse
      • Unigene: 498178 Human
      • Unigene: 37638 Mouse
      • Alternative names

        • Actin binding protein antibody
        • Actinin alpha 2 antibody
        • ACTN 2 antibody
        • ACTN2 antibody
        • ACTN2_HUMAN antibody
        • Alpha actinin 2 antibody
        • Alpha actinin skeletal muscle antibody
        • Alpha actinin skeletal muscle isoform 2 antibody
        • Alpha-actinin skeletal muscle isoform 2 antibody
        • Alpha-actinin-2 antibody
        • CMD1AA antibody
        • F actin cross linking protein antibody
        • F-actin cross-linking protein antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 103 kDa
        Observed band size: 103 kDa



        Blocking and dilution buffer: 5% NFDM /TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

        ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.

        Lane 1 (input): HepG2 whole cell lysate (10µg)

        Lane 2 (+): ab68167 + HepG2 whole cell lysate.

        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.

        For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.



        All lanes :

        Lane 1 : HepG2 whole cell lysate at 10 µg
        Lane 2 : ab68167 + HepG2 whole cell lysate at 10 µg
        Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate

        Observed band size: 103 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)

        Lane 1 : Human skeletal muscle lysate
        Lane 2 : Rat heart lysate
        Lane 3 : Human heart lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 103 kDa
        Observed band size: 103 kDa

      • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 103 kDa
        Observed band size: 103 kDa



        Blocking and dilution buffer: 5% NFDM /TBST.

      • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)

        Lane 1 : Mouse brain tissue lysate
        Lane 2 : Rat brain tissue lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 103 kDa
        Observed band size: 103 kDa



        Blocking and dilution buffer: 5% NFDM /TBST.

      • Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
        Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (29)

      Publishing research using ab68167? Please let us know so that we can cite the reference in this datasheet.

      ab68167 has been referenced in 29 publications.

      • Taniguchi Y  et al. Tissue suction-mediated gene transfer to the beating heart in mice. PLoS One 15:e0228203 (2020). PubMed: 32027678
      • Cardoso AC  et al. Mitochondrial Substrate Utilization Regulates Cardiomyocyte Cell Cycle Progression. Nat Metab 2:167-178 (2020). PubMed: 32617517
      • Tousley A  et al. Rac1 Activity Is Modulated by Huntingtin and Dysregulated in Models of Huntington's Disease. J Huntingtons Dis 8:53-69 (2019). PubMed: 30594931
      • Wang HB  et al. Cordycepin ameliorates cardiac hypertrophy via activating the AMPKa pathway. J Cell Mol Med 23:5715-5727 (2019). PubMed: 31225721
      • Findlay AR  et al. Lithium chloride corrects weakness and myopathology in a preclinical model of LGMD1D. Neurol Genet 5:e318 (2019). PubMed: 31123706
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-8 of 8 Abreviews or Q&A

      Western blot abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Heart)
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Loading amount
      30 µg
      Specification
      Heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 10 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Neonatal cardiomyocytes)
      Permeabilization
      Yes - 0.2% tritonX-100
      Specification
      Neonatal cardiomyocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Methanol
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 18 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (cardiomyocytes)
      Permeabilization
      Yes - 0.2% Trition
      Specification
      cardiomyocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Methanol
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 09 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (mouse heart section)
      Permeabilization
      Yes - tween 20/PBS
      Specification
      mouse heart section
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 06 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Heart)
      Permeabilization
      Yes - Tween-20
      Specification
      Heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 17 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (Heart)
      Permeabilization
      Yes - Triton 0.1%
      Specification
      Heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 07 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (heart)
      Permeabilization
      Yes - 0.3% Triton X
      Specification
      heart
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 20%
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted May 11 2015

      Western blot

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      4 µg
      Gel Running Conditions
      Reduced Denaturing (12% gel)
      Sample
      Mouse Cell lysate - whole cell (mouse cardiac cells. Cardiomyocytes and non-cardio)
      Specification
      mouse cardiac cells. Cardiomyocytes and non-cardio
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 23 2013

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