Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)
- Datasheet
- Specific References (14)
- Protocols
Overview
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Product nameAnti-Sarcomeric Alpha Actinin antibody [EP2529Y]
See all Sarcomeric Alpha Actinin primary antibodies -
DescriptionRabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP2529Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Positive Controls
- Rat brain normal tissue lysate - total protein (ab29475)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse heart tissue lysate - total protein (0 days) (ab7193)
- Mouse heart tissue lysate - total protein (14 days) (ab7194)
- Mouse heart tissue lysate - total protein (7 days) (ab7196)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab68167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). | |
| IP | 1/20 - 1/40. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
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Tissue specificityExpressed in both skeletal and cardiac muscle.
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Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
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Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
- Information by UniProt
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Database links
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
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Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-6-ab68167WB1.jpg)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-4-ab68167IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-7-ab68167WB2.jpg)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-5-ab68167IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-8-ab68167WB3.jpg)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167-3-ab68167IP1.jpg)
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab68167 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.
For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167wb.gif)
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat heart lysate
Lane 3 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)](https://www.abcam.com/ps/products/68/ab68167/Images/ab68167ihc2 copy.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.
References
This product has been referenced in:
- Zhang X et al. Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKa/Smad3 signaling. Cell Death Dis 9:102 (2018). Read more (PubMed: 29367637) »
- Beekman C et al. Use of capillary Western immunoassay (Wes) for quantification of dystrophin levels in skeletal muscle of healthy controls and individuals with Becker and Duchenne muscular dystrophy. PLoS One 13:e0195850 (2018). Read more (PubMed: 29641567) »
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