Name: Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]
Brand: RabMAb
SKU: ab68167
Rating: 5 (4 reviews)

Product name

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]
See all Sarcomeric Alpha Actinin primary antibodies
Description

Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2529Y
Isotype

IgG
Research areas

Alternative Versions
- Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

Our Abpromise guarantee covers the use of ab68167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IP		1/20 - 1/40.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Application notes

Is unsuitable for Flow Cyt or ICC/IF.

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificity

Expressed in both skeletal and cardiac muscle.
Involvement in disease

Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similarities

Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localization

Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
Target information above from: UniProt accession P35609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
- ACTN2 antibody
- ACTN2_HUMAN antibody
- Alpha actinin 2 antibody
- Alpha actinin skeletal muscle antibody
- Alpha actinin skeletal muscle isoform 2 antibody
- Alpha-actinin skeletal muscle isoform 2 antibody
- Alpha-actinin-2 antibody
- CMD1AA antibody
- F actin cross linking protein antibody
- F-actin cross-linking protein antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab68167 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.
For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)

Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat heart lysate
Lane 3 : Human heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and dilution buffer: 5% NFDM /TBST.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and dilution buffer: 5% NFDM /TBST.

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Datasheet

This product has been referenced in:

Zhang X et al. Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKa/Smad3 signaling. Cell Death Dis 9:102 (2018). Read more (PubMed: 29367637) »
Beekman C et al. Use of capillary Western immunoassay (Wes) for quantification of dystrophin levels in skeletal muscle of healthy controls and individuals with Becker and Duchenne muscular dystrophy. PLoS One 13:e0195850 (2018). Read more (PubMed: 29641567) »

See all 16 Publications for this product

Publishing research using ab68167? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Heart)

Permeabilization

Yes - Tween-20

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 17 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Rat Tissue sections (Heart)

Permeabilization

Yes - Triton 0.1%

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 07 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (heart)

Permeabilization

Yes - 0.3% Triton X

Specification

heart

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 20%

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted May 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Loading amount

4 µg

Gel Running Conditions

Reduced Denaturing (12% gel)

Sample

Mouse Cell lysate - whole cell (mouse cardiac cells. Cardiomyocytes and non-cardio)

Specification

mouse cardiac cells. Cardiomyocytes and non-cardio

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Overview

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Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

Western blot