Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts
- Sensitivity: 1 µM
Product nameSarcosine Assay Kit
Sample typeCell culture supernatant, Milk, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity> 1 µM
Range1 µM - 10000 µM
Assay time1h 00m
Abcam's Sarcosine Assay Kit provides an accurate, convenient measure of sarcosine in variety biological samples. In the assay, sarcosine is specifically oxidized to generate a product that converts a colorless probe to a product with intense red color (λmax = 570 nm) and which is also highly fluorescent (Ex/Em = 538/587 nm). Sarcosine is therefore easily detected by either colorimetric or fluorometric methods with detection range 1-10000 µM.
Visit our FAQs page for tips and troubleshooting.
Sarcosine, a natural amino acid, plays important roles as intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine, etc. Detection of sarcosine level has wide applications in research and development.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Sarcosine Assay Buffer WM 1 x 30ml Sarcosine Enzyme Mix (Lyophilized) Green 1 vial Sarcosine Probe (in DMSO, anhydrous) Red 1 vial Sarcosine Standard (10 µmol) (Lyophilized) Yellow 1 vial
RelevanceSarcosine (N-methylglycine) is an intermediate and byproduct in the synthesis and degradation of glycine. Sarcosine is metabolized to glycine by sarcosine dehydrogenase, while glycine-N-methyl transferase generates sarcosine from glycine. Sarcosine is a natural amino acid found in muscles and other body tissues and plays important role as an intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine.
- N methylglycine
Note: Urine samples do not work well with the assay due to sample interferences.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ab65338 has been referenced in 1 publication.
- Barfeld SJ et al. c-Myc Antagonises the Transcriptional Activity of the Androgen Receptor in Prostate Cancer Affecting Key Gene Networks. EBioMedicine 18:83-93 (2017). PubMed: 28412251