Overview

  • Product name
  • Description
    Rabbit polyclonal to SARM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • Daudi cells

Properties

Applications

Our Abpromise guarantee covers the use of ab17812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 80 kDa.
ICC/IF Use a concentration of 2 µg/ml.

Target

  • Function
    Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation.
  • Tissue specificity
    Predominantly expressed in kidney and liver. Expressed at lower level in placenta.
  • Sequence similarities
    Contains 2 SAM (sterile alpha motif) domains.
    Contains 1 TIR domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36296 antibody
    • KIAA0524 antibody
    • MyD88-5 antibody
    • SAM and ARM-containing protein antibody
    • SAM domain-containing protein 2 antibody
    • SAMD2 antibody
    • SARM 1 antibody
    • SARM1 antibody
    • SARM1_HUMAN antibody
    • Sterile alpha and Armadillo repeat protein antibody
    • sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila antibody
    • sterile alpha and HEAT/Armadillo motifs-containing protein antibody
    • sterile alpha and TIR motif containing 1 antibody
    • Sterile alpha and TIR motif-containing protein 1 antibody
    • Sterile alpha and TIR motifs-containing protein 1 antibody
    • Sterile alpha motif domain-containing protein 2 antibody
    • Tir-1 homolog antibody
    see all

Images

  • Western Blot of Daudi cell lysate labeling SARM with Anti-SARM antibody (ab17812) at (1) 0.5 and (2) 1µg/ml.
  • Lane 1 : Anti-SARM antibody (ab17812) at 0.5 µg/ml
    Lane 2 : Anti-SARM antibody (ab17812) at 1 µg/ml
    Lane 3 : Anti-SARM antibody (ab17812) at 2 µg/ml

    All lanes : Daudi cell lysate

    Predicted band size: 80 kDa
    Observed band size: 80 kDa



    Western blot analysis of SARM (79 kDa) in Daudi cell lysates with ab17812 at (A) 0.5, (B) 1, and (C) 2 µg/ml.
  • ab17812 at 2µg/ml staining of SARM in Daudi cells by ICC/IF 

References

This product has been referenced in:
  • Liu M  et al. Overexpression of miR-422a inhibits cell proliferation and invasion, and enhances chemosensitivity in osteosarcoma cells. Oncol Rep 36:3371-3378 (2016). Read more (PubMed: 27779704) »
  • de Vos AF  et al. In vivo lipopolysaccharide exposure of human blood leukocytes induces cross-tolerance to multiple TLR ligands. J Immunol 183:533-42 (2009). WB ; Human . Read more (PubMed: 19542464) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fly head)
Specification
Fly head
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 10 2015

Answer

Thank you for contacting us. We do not know what the identity of the additional bands on the WB image are. They may be non-specific bands. The immunogen used to generate the antibody is chosen to be unique, so that it is unlikely to react with other proteins. The article ciuted on the website did use the antibody for a Western Blot in figure 7, but they quantified the blot with densitometry which is why you did not see the image itself. We do not currently have any production plans for another SARM antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (RAJI B-Cells)
Loading amount
20 µg
Specification
RAJI B-Cells
Gel Running Conditions
Reduced Denaturing (12% Tris-HEPES-SDS Gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 14 2009

Answer

Thank you for your enquiry. I have been informed that unfortunately the homology is only 61%, therefore we predict that there is little chance for cross reaction with zebrafish protein; there are 6 highly conserved residues next to each other, enough for an epitope, but the chance is very small. I'm sorry we don't seem to have other antibodies for Sarm.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (monocytes)
Loading amount
20 µg
Specification
monocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Feb 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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