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Dear Support Staff, Regarding Anti-SARM (ab17812) antibody, I have a few querry to clarify before I could proceed for the purchase. First thing is Why are there many non-specific bands in the Western blot for the detection of SARM? Secondly, Is the figure that has been shown on the website a published data/work? And the reference that the website gave for the ab17812 does not show any western blot figure on their published work, so, How could we be assured that the antibody really detects the desired protein SARM. Could you specifically tell from which region of SARM the antibody (ab17812) has been generated? And lastly, has there been any instance that your company thought of generating anti-SARM antibody raised from the peptide region of N-terminal portion of the particular protein, if so When we would we expect it to commercially available?
Asked on Nov 23 2011
Thank you for contacting us. We do not know what the identity of the additional bands on the WB image are. They may be non-specific bands. The immunogen used to generate the antibody is chosen to be unique, so that it is unlikely to react with other proteins. The article ciuted on the website did use the antibody for a Western Blot in figure 7, but they quantified the blot with densitometry which is why you did not see the image itself. We do not currently have any production plans for another SARM antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 23 2011