Overview

  • Product name

  • Description

    Rabbit polyclonal to SARM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SARM (C terminal). The exact sequence is proprietary.
    Database link: Q6SZW1

  • Positive control

    • WB: A431, HeLa and HepG2 whole cell extracts; Mouse and rat brain extracts. IHC-P: Rat kidney tissue. ICC/IF: Rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab226930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 79 kDa.

Target

  • Function

    Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation.
  • Tissue specificity

    Predominantly expressed in kidney and liver. Expressed at lower level in placenta.
  • Sequence similarities

    Contains 2 SAM (sterile alpha motif) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36296 antibody
    • KIAA0524 antibody
    • MyD88-5 antibody
    • SAM and ARM-containing protein antibody
    • SAM domain-containing protein 2 antibody
    • SAMD2 antibody
    • SARM 1 antibody
    • SARM1 antibody
    • SARM1_HUMAN antibody
    • Sterile alpha and Armadillo repeat protein antibody
    • sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila antibody
    • sterile alpha and HEAT/Armadillo motifs-containing protein antibody
    • sterile alpha and TIR motif containing 1 antibody
    • Sterile alpha and TIR motif-containing protein 1 antibody
    • Sterile alpha and TIR motifs-containing protein 1 antibody
    • Sterile alpha motif domain-containing protein 2 antibody
    • Tir-1 homolog antibody
    see all

Images

  • All lanes : Anti-SARM antibody (ab226930) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-SARM antibody (ab226930) at 1/500 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded rat kidney tissue stained for SARM with ab226930 at 1/400 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed rat E18 primary cortical neurons stained for SARM (green) using ab226930 at 1/500 dilution in ICC/IF.

    The nuclear counter stain is DAPI (blue). Beta-Tubulin is detected with a beta-Tubulin antibody (red).

References

ab226930 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226930.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up