Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus
- Suitable for: ELISA, IHC-P
- Isotype: IgG
Overview
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Product name
Anti-SARS-CoV-2 envelope protein antibody - Coronavirus -
Description
Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-Pmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Synthetic peptide within Human coronavirus SARS-CoV-2 envelope protein aa 1-50 (N terminal). The exact sequence is proprietary. 10 amino acid peptide
Database link: QHD43418.1
(Peptide available asab273064) -
General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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ELISA
Use a concentration of 1 µg/ml. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of COVID-19 patient lung tissue labeling SARS-CoV-2 envelope protein with ab272503 at 1 µg/mL. Tissue was blocked with 10% serum for 1 hour at room temperature. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Samples were incubated with the primary antibody overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/250). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Strong positive signal observed in macrophage cells of COVID-19 patient lung but not in non-COVID-19 patient lung.
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ELISA - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)A direct ELISA was performed using antigen or control peptide as coating antigen and ab272503 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 32ng/ml to 2000 ng/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272503 has not yet been referenced specifically in any publications.