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  1. Link

    sars-cov-2-envelope-protein-antibody-coronavirus-ab272503.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
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Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)

  • Datasheet
  • SDS
Reviews (3) Submit a question

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)
  • ELISA - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)

Key features and details

  • Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus
  • Suitable for: ELISA, IHC-P
  • Isotype: IgG

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Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
Peptide
Human coronavirus SARS-CoV-2 envelope protein peptide (ab273064)

View more associated products

Overview

  • Product name

    Anti-SARS-CoV-2 envelope protein antibody - Coronavirus
    See all SARS-CoV-2 envelope protein primary antibodies
  • Description

    Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: SARS-CoV-2
  • Immunogen

    Synthetic peptide within Human coronavirus SARS-CoV-2 envelope protein aa 1-50 (N terminal). The exact sequence is proprietary. 10 amino acid peptide
    Database link: QHD43418.1
    (Peptide available as ab273064)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human coronavirus SARS-CoV-2 envelope protein peptide (ab273064)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ELISA
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of COVID-19 patient lung tissue labeling SARS-CoV-2 envelope protein with ab272503 at 1 µg/mL. Tissue was blocked with 10% serum for 1 hour at room temperature. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Samples were incubated with the primary antibody overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/250). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    Strong positive signal observed in macrophage cells of COVID-19 patient lung but not in non-COVID-19 patient lung.

  • ELISA - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)
    ELISA - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)

    A direct ELISA was performed using antigen or control peptide as coating antigen and ab272503 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 32ng/ml to 2000 ng/ml.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab272503? Please let us know so that we can cite the reference in this datasheet.

ab272503 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SARS-CoV-2 envelope protein antibody - Coronavirus

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Permeabilization
Yes - Triton X-100
Specification
A549
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 31 2022

Immunoprecipitation abreview for Anti-SARS-CoV-2 envelope protein antibody - Coronavirus

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Cat Cell lysate - other (Serum with 2 ug IgG)
Total protein in input
2 µg
Immuno-precipitation step
Other - Protein A/G coated Dynabeads
Specification
Serum with 2 ug IgG
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Stefan Keller

Verified customer

Submitted May 28 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-SARS-CoV-2 envelope protein antibody - Coronavirus

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
SARS-CoV-2 Cell (VERO-E6 (african green monkey))
Permeabilization
Yes - 0.5% Saponin
Specification
VERO-E6 (african green monkey)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Harry Wodrich

Verified customer

Submitted Jul 22 2020

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