Recombinant Anti-SARS-COV-2 nsp3 antibody [EPR24842-84] (ab283958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24842-84] to SARS-COV-2 nsp3
- Suitable for: WB, ELISA
Related conjugates and formulations
Overview
-
Product name
Anti-SARS-COV-2 nsp3 antibody [EPR24842-84]
See all SARS-COV-2 nsp3 primary antibodies -
Description
Rabbit monoclonal [EPR24842-84] to SARS-COV-2 nsp3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Elisa: Recombinant SARS-COV-2 NSP3 protein (His-tag) WB: recombinant SARS-COV-2 NSP3 protein (His-tag)
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24842-84 -
Isotype
IgG
Associated products
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 21 kDa.
|
ELISA |
Use a concentration of 2e-005 - 1 µg/ml.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 21 kDa. |
ELISA
Use a concentration of 2e-005 - 1 µg/ml. |
Images
-
All lanes: Anti-SARS-CoV2 NSP3 antibody (ab283958) at 1/1000 dilution
Lanes 1-2: Recombinant SARS-CoV2 NSP3 protein (His tagged) at 0.4 µg
Lanes 1: Red – loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 21 kDa.
Lanes 2: Green – ab283958 observed at 21 kDa
ab283958 was shown to bind specifically to SARS-CoV-2 NSP3 protein in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T)
-
Indirect ELISA showing primary antibody ab283958 binding to recombinant SARS-COV-2 NSP3 protein (His-tag). Plates were coated with recombinant SARS-COV-2 NSP3 protein (His-tag) and recombinant SARS-COV-2 NSP9 protein (His-tag) at 1000 ng/ml. Binding of ab283958 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).
Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab283958 has not yet been referenced specifically in any publications.