Key features and details
- Rabbit polyclonal to SARS-CoV-2 nucleocapsid protein
- Suitable for: WB, IHC
- Isotype: IgG
Product nameAnti-SARS-CoV-2 nucleocapsid protein antibody
See all SARS-CoV-2 nucleocapsid protein primary antibodies
DescriptionRabbit polyclonal to SARS-CoV-2 nucleocapsid protein
Tested applicationsSuitable for: WB, IHCmore details
Species reactivityReacts with: SARS-CoV-2
Synthetic peptide within Human SARS-CoV-2 nucleocapsid protein aa 1-100. The exact sequence is proprietary.
- WB: Recombinant Nucleoprotein from SARS-CoV-2. IHC: Human lung tissue.
Tick: Tested and Guaranteed to work X: Will not work —: No data
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.0
Preservative: 0.02% Sodium azide
Constituents: 0.16% Tris HCl, 0.87% Sodium chloride
Concentration information loading...
Purity>95% by SDS-PAGE
Our Abpromise guarantee covers the use of ab273167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC||Use a concentration of 2 - 10 µg/ml.|
RelevanceNucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
- 2019-nCoV nucleocapsid protein antibody
- N antibody
- NC antibody
Immunohistochemistry analysis of patient lung biopsy samples infected with SARS-CoV-2 (A) and diagnosed with non-specific lung infection (B) labelling SARS-CoV-2 Nucleoprotein (dark brown) using ab273167 at 10 μg/ml, followed by HRP-conjugated Goat Anti-Rabbit IgG. Magnification (x400). Cells in Fig (A) stained dark brown confirm SARS-CoV-2 infection.
Anti-SARS-CoV-2 nucleocapsid protein antibody (ab273167) at 2 µg/ml + Purified recombinant Nucleoprotein from SARS-CoV-2
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab273167 has not yet been referenced specifically in any publications.