Product nameAnti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus
See all SARS-CoV-2 spike glycoprotein primary antibodies
DescriptionRabbit polyclonal to SARS-CoV-2 spike glycoprotein - Coronavirus
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: SARS-CoV-2
- WB: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein, cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab272504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Can be blocked with Human coronavirus SARS-CoV-2 spike glycoprotein peptide (ab273063).|
All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) at 1 µg/ml
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : HEK-293 overexpressing SARS-CoV-2 spike glycoprotein, cell lysate
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/1000 dilution
Primary incubation for 1 hour at room temperature.
Blocking and dilution buffer: 5% NFDM/TBST.
A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to 1000 ng/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272504 has not yet been referenced specifically in any publications.