Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric
- Suitable for: ELISA
- Reacts with: Human coronavirus
Product nameAnti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric
See all SARS-CoV-2 spike glycoprotein primary antibodies
DescriptionMouse monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Human coronavirus
Recombinant fragment corresponding to Human SARS-CoV-2 spike glycoprotein.
Database link: P0DTC2
Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.
As the Fc region is human, an anti-Human secondary antibody should be used for detection.
Tick: Tested and Guaranteed to work X: Will not work —: No data
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Constituents: 0.03% Proclin 300, 50% Glycerol, PBS
Concentration information loading...
Purity>90% by SDS-PAGE
Purification notesScreened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells.
Our Abpromise guarantee covers the use of ab272854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/10000 - 1/50000.|
Binding Activity of ab272854 with SARS-CoV-2 spike glycoprotein.
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 spike glycoprotein at 2 μg/ml can bind ab272854. The EC50 of ab272854 is 36.79-48.87 ng/ml.
Binding Activity of ab272854 with SARS-CoV-2-S1-RBD
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind ab272854, the EC50 of SARS-CoV-2-S Antibody is 19.60-39.42 ng/ml.
Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 23.32 nM.
Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 2.38 µg/mL.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272854 has not yet been referenced specifically in any publications.