Key features and details
- Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD
- Suitable for: WB, ICC
- Reacts with: Recombinant fragment
- Isotype: IgG1
Product nameAnti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1]
See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies
DescriptionMouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD
Tested applicationsSuitable for: WB, ICCmore details
Species reactivityReacts with: Recombinant fragment, SARS-CoV-2
Predicted to work with: Human coronavirus
Recombinant fragment corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD.
Database link: P0DTC2
- WB: HEK-293 cells transfected with the SARS-CoV2 binding domain. ICC: HEK-293 cells transfected with S-protein segment.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Tissue Culture supernatant.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/3000.
1/1000 - 1/3000.
HEK-293 cells transfected with the DNA encoding the S-protein segment stained for spike glycoprotein using ab277624 (green) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.
Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624) at 1/3000 dilution
Lanes 1 & 5 : Molecular weight ladder
Lane 2 : crude homogenate of untransfected control HEK-293 cells
Lane 3 : homogenate of HEK-293 cells expressing SARS-CoV2-bd
Lane 4 : homogenate of HEK-293 cells expressing GFP-SARS-CoV2-bd fusion
Lane 6 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 1 µg
Lane 7 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 0.1 µg
Additional bands at: 60 kDa (possible Loading Control)
Western blot analysis of cells transfected with DNA encoding the S-protein ACE2 binding site in PROT-SARS-CoV2-bd which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP using ab277624 (green) at 1/3000 dilution. The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277624 has not yet been referenced specifically in any publications.