Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624)
![Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)](https://www.abcam.com/ps/products/277/ab277624/Images/ab277624-434726-AntiSpike-glycoprotein-antibody-2G1-ICC-HEK293-cells-transfected-with-DNA-encoding-the-Sprotein-segment.JPG "Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)")
Key features and details
- Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD
- Suitable for: WB, ICC
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1]
See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies -
Description
Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Recombinant fragment, SARS-CoV-2
Predicted to work with: Human coronavirus
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Immunogen
Recombinant fragment corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD.
Database link: P0DTC2 -
Positive control
- WB: HEK-293 cells transfected with the SARS-CoV2 binding domain. ICC: HEK-293 cells transfected with S-protein segment.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
2G1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/3000.
|
|
| ICC |
1/1000.
|
| Notes |
|---|
|
WB
1/1000 - 1/3000. |
|
ICC
1/1000. |
Images
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Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)
HEK-293 cells transfected with the DNA encoding the S-protein segment stained for spike glycoprotein using ab277624 (green) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.
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![Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)](https://www.abcam.com/ps/products/277/ab277624/Images/ab277624-434725-AntiSpike-glycoprotein-antibody-2G1-WB-HEK293-cells-transfected-with-SARSCoV2-binding-domain.JPG)
Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624) at 1/3000 dilution
Lanes 1 & 5 : Molecular weight ladder
Lane 2 : crude homogenate of untransfected control HEK-293 cells
Lane 3 : homogenate of HEK-293 cells expressing SARS-CoV2-bd
Lane 4 : homogenate of HEK-293 cells expressing GFP-SARS-CoV2-bd fusion
Lane 6 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 1 µg
Lane 7 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 0.1 µg
Additional bands at: 60 kDa (possible Loading Control)Western blot analysis of cells transfected with DNA encoding the S-protein ACE2 binding site in PROT-SARS-CoV2-bd which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP using ab277624 (green) at 1/3000 dilution. The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab277624 has not yet been referenced specifically in any publications.
