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  1. Link

    sars-cov-2-spike-glycoprotein-rbd-antibody-2g1-ab277624.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
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Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624)

  • Datasheet
  • SDS
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Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)
  • Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)

Key features and details

  • Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD
  • Suitable for: WB, ICC
  • Reacts with: Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1]
    See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies
  • Description

    Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Recombinant fragment, SARS-CoV-2
    Predicted to work with: Human coronavirus
  • Immunogen

    Recombinant fragment corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD.
    Database link: P0DTC2

  • Positive control

    • WB: HEK-293 cells transfected with the SARS-CoV2 binding domain. ICC: HEK-293 cells transfected with S-protein segment.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from Tissue Culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2G1
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab277624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/3000.
ICC
1/1000.
Notes
WB
1/1000 - 1/3000.
ICC
1/1000.

Images

  • Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)
    Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)

    HEK-293 cells transfected with the DNA encoding the S-protein segment stained for spike glycoprotein using ab277624 (green) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.

  • Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)
    Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)
    Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624) at 1/3000 dilution

    Lanes 1 & 5 : Molecular weight ladder
    Lane 2 : crude homogenate of untransfected control HEK-293 cells
    Lane 3 : homogenate of HEK-293 cells expressing SARS-CoV2-bd
    Lane 4 : homogenate of HEK-293 cells expressing GFP-SARS-CoV2-bd fusion
    Lane 6 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 1 µg
    Lane 7 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 0.1 µg

    Additional bands at: 60 kDa (possible Loading Control)



    Western blot analysis of cells transfected with DNA encoding the S-protein ACE2 binding site in PROT-SARS-CoV2-bd which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP using ab277624 (green) at 1/3000 dilution. The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution. 

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab277624? Please let us know so that we can cite the reference in this datasheet.

ab277624 has not yet been referenced specifically in any publications.

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