Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628)
Key features and details
- Mouse monoclonal [5G8] to SARS-CoV-2 Spike Glycoprotein RBD
- Suitable for: WB, ICC
- Isotype: IgG1
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8]
See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies -
Description
Mouse monoclonal [5G8] to SARS-CoV-2 Spike Glycoprotein RBD -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: SARS-CoV-2
Predicted to work with: Human coronavirus -
Immunogen
Recombinant fragment (His-tag) corresponding to Escherichia coli SARS-CoV-2 Spike Glycoprotein RBD aa 300-550 (N terminal).
Database link: P0DTC2 -
Positive control
- WB: HEK-293 cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA, cells transfected with pCI-Neo-GFP vector expression construct containing containing the SARS-CoV2-bd cDNA. ICC: HEK-293 cells transfected with pCI-Neo-Mod vector including SARS-CoV2-bd cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
5G8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/3000.
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ICC |
1/1000 - 1/5000.
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Notes |
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WB
1/1000 - 1/3000. |
ICC
1/1000 - 1/5000. |
Images
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HEK-293 cells transfected with pCI-Neo-Mod vector including SARS-CoV2-bd cDNA stained for Spike glycoprotein using ab277628 (Red) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with Hoechst staining.
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Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628) at 1/3000 dilution
Lanes 1 & 5 : Molecular weight ladder
Lane 2 : Non-transfected HEK-293 cells
Lane 3 : HEK-293 cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA
Lane 4 : HEK-293 cells transfected with pCI-Neo-GFP vector expression construct containing the SARS-CoV2-bd cDNA
Lane 6 : Full length recombinant SARS-CoV2 S-protein expressed in HEK293 cells at 1 µg
Lane 7 : Full length recombinant SARS-CoV2 S-protein expressed in HEK293 cells at 0.1 µg
Additional bands at: 60 kDa (possible Loading Control)The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution revealing a single band at 60kDa in both transfected and non-transfected cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab277628 has been referenced in 2 publications.
- Fraser BJ et al. Structure and activity of human TMPRSS2 protease implicated in SARS-CoV-2 activation. Nat Chem Biol 18:963-971 (2022). PubMed: 35676539
- Zhang H et al. APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients. Signal Transduct Target Ther 7:261 (2022). PubMed: 35915083