Key features and details
- Mouse monoclonal [5G8] to SARS-CoV-2 Spike Glycoprotein RBD
- Suitable for: WB, ICC
- Isotype: IgG1
Product nameAnti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8]
See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies
DescriptionMouse monoclonal [5G8] to SARS-CoV-2 Spike Glycoprotein RBD
Tested applicationsSuitable for: WB, ICCmore details
Predicted to work with: Human coronavirus
Recombinant fragment corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD.
Database link: P0DTC2
- WB: HEK-293 cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA, cells transfected with pCI-Neo-GFP vector expression construct containing containing the SARS-CoV2-bd cDNA. ICC: HEK-293 cells transfected with pCI-Neo-Mod vector including SARS-CoV2-bd cDNA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Tissue Culture supernatant.
Our Abpromise guarantee covers the use of ab277628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000.|
|ICC||1/1000 - 1/5000.|
HEK-293 cells transfected with pCI-Neo-Mod vector including SARS-CoV2-bd cDNA stained for Spike glycoprotein using ab277628 (Red) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with Hoechst staining.
Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628) at 1/3000 dilution
Lanes 1 & 5 : Molecular weight ladder
Lane 2 : Non-transfected HEK-293 cells
Lane 3 : HEK-293 cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA
Lane 4 : HEK-293 cells transfected with pCI-Neo-GFP vector expression construct containing the SARS-CoV2-bd cDNA
Lane 6 : Full length recombinant SARS-CoV2 S-protein expressed in HEK293 cells at 1 µg
Lane 7 : Full length recombinant SARS-CoV2 S-protein expressed in HEK293 cells at 0.1 µg
Additional bands at: 60 kDa (possible Loading Control)
The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution revealing a single band at 60kDa in both transfected and non-transfected cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277628 has not yet been referenced specifically in any publications.