Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK293T)
Total protein in input
1e+007 cells
Immuno-precipitation step
Protein G
Specification
HEK293T
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 150 mM Nacl, 50 mM Tris, %1 NP-40
Dilution
1/1000
Western Blot antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Goat Anti Rabbit IRDye 680RD
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Additional data
Additional Notes
I used SARS-CoV-2 (2019-nCoV) Spike RBD Antibody from Sino biological as primary antibody for western blotting, since this antibody does not work in WB.
I write 10 million cell because I didn't get that part nicely. I lysed 10 cm plate cells in 1 ml lysis buffer. Used 900 ul of it with pull-down assay. I used 1,5 ug antibody and 0,3 mg of Protein G Dynomobeads for 1 lysate.
Because both antibodies are Rabbit we have bands for heavy and light chains.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Hasan Ugurlu
Verified customer
Submitted Jun 29 2020