Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL237] to SARS-CoV-2 Spike Glycoprotein S2 - BSA and Azide free
- Suitable for: WB, IHC-P, Sandwich ELISA, ICC/IF
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free
See all SARS-CoV-2 Spike Glycoprotein S2 primary antibodies -
Description
Rabbit monoclonal [HL237] to SARS-CoV-2 Spike Glycoprotein S2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Sandwich ELISA, ICC/IFmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Synthetic peptide within Human coronavirus SARS-CoV-2 Spike Glycoprotein S2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Carrier-protein conjugated synthetic peptide SARS-CoV-2 (strain Wuhan-Hu-1)
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Positive control
- IHC-P: SARS-CoV-2 (COVID-19) Spike transfected 293T cell ICC/IF: SARS-CoV-2 (COVID-19) Spike transfected 293T cells. WB: SARS-CoV-2 (COVID-19) Spike transfected 293T cells. ELISA: Recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL237 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Sandwich ELISA |
Use a concentration of 1 µg/ml.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Sandwich ELISA
Use a concentration of 1 µg/ml. |
ICC/IF
1/100 - 1/1000. |
Images
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All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : SARS-CoV Spike transfected 293T whole cell extract
Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
Lane 4 : MERS Spike transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
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Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S2 stained (green) by ab281312 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue).
Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.
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Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike S2 stained (green) by ab281312 at 1/1000 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).
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Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at 5 µg/mL and ab281312 as detection antibody at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution and used to detect the primary antibody.
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All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab281312 has been referenced in 1 publication.
- Boix-Besora A et al. Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2. Vaccines (Basel) 10:N/A (2022). PubMed: 35214708