Overview

Description

  • Nature
    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab39839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS, pH 7.2

General Info

  • Alternative names
    • E protein
    • Envelope protein
    • Envelope small membrane protein
    • protein E
    • protein sM
    • sM protein
    see all
  • Relevance
    A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. The evelope protein is a component of the viral envelope that plays a central role in virus morphogenesis and assembly. It may be sufficient to form virus like particles.
  • Cellular localization
    Cell Membrane; single pass membrane protein.

References

ab39839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. I am sorry to confirm we do not have any SARS antibodies that are tested in sandwich ELISA (sELISA). SARS E2 antibody [4A6C9] (ab31951) and SARS Nucleoprotein antibody [BDI186] (ab28169) are tested in indirect ELISA only. I can suggest to consider trying one of them in an indirect ELISA method. Sandwich ELISA procedures can be difficult to optimise and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. I am sorry we have no suitably tested antibodies for sELISA on this occasion. I can suggest having a look on the Biocompare website. They have an excellent antibody search facility which includes a lot of suppliers. Regrettably, I am also not able to confirm that the ab28169 is raised against the SARS nucleoprotein, so will not detect the ab39839 E2 protein peptide. However, the ab39839 peptide has been specifically used as the immunogen for ab31951 SARS E2 antibody [4A6C9], so these can be used together in indirect ELISA. I hope this will be helpful. Should you have any further questions, please do not hesitate to contact us. Good luck with your research.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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