Overview

  • Product name
    Anti-SARS E2 antibody [4A6C9]
  • Description
    Mouse monoclonal [4A6C9] to SARS E2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human coronavirus
  • Immunogen

    Ni-NTA purified recombinant SARS-E2 glycoprotein precursor expressed in E. Coli strain BL21 (DE3)

  • Positive control
    • SARS E2 Recombinant protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    4A6C9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 36 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The complete sequence of the SARS virus release the coronavirus contains 25 open reading frames. SARS E2 glycoprotein precursor is a 139kDa glycoprotein. It contains a superantigen between residues 690 through 1050 which has relationship to T-cell Receptor alpha-beta V chain protein.

Images

References

ab31951 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. I am sorry to confirm we do not have any SARS antibodies that are tested in sandwich ELISA (sELISA). SARS E2 antibody [4A6C9] (ab31951) and SARS Nucleoprotein antibody [BDI186] (ab28169) are tested in indirect ELISA only. I can suggest to consider trying one of them in an indirect ELISA method. Sandwich ELISA procedures can be difficult to optimise and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. I am sorry we have no suitably tested antibodies for sELISA on this occasion. I can suggest having a look on the Biocompare website. They have an excellent antibody search facility which includes a lot of suppliers. Regrettably, I am also not able to confirm that the ab28169 is raised against the SARS nucleoprotein, so will not detect the ab39839 E2 protein peptide. However, the ab39839 peptide has been specifically used as the immunogen for ab31951 SARS E2 antibody [4A6C9], so these can be used together in indirect ELISA. I hope this will be helpful. Should you have any further questions, please do not hesitate to contact us. Good luck with your research.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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