Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951)

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Overview

  • Product name

    Anti-SARS E2 antibody [4A6C9] - Coronavirus
    See all SARS E2 primary antibodies

  • Description

    Mouse monoclonal [4A6C9] to SARS E2 - Coronavirus

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human coronavirus

  • Immunogen

    Ni-NTA purified recombinant SARS-E2 glycoprotein precursor expressed in E. Coli strain BL21 (DE3)

  • Positive control

    • SARS E2 Recombinant protein.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    4A6C9

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 36 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The complete sequence of the SARS virus release the coronavirus contains 25 open reading frames. SARS E2 glycoprotein precursor is a 139kDa glycoprotein. It contains a superantigen between residues 690 through 1050 which has relationship to T-cell Receptor alpha-beta V chain protein.

Images

  • Western blot - Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951)
    Western blot - Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951)
    Lane 1 : MW Marker
    Lane 2 : Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951) at 1/2000 dilution

    Lane 2 : SARS E2 Recombinant protein

    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

References

ab31951 has not yet been referenced specifically in any publications.

Publishing research using ab31951? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

ab39839  SARS E protein peptide    Could it be used with Anti-SARS E2 antibody [4A6C9] (ab31951) and Anti-SARS Nucleoprotein antibody [BDI186] (ab28169) to do sandwich assay? I want one antigen (SARS) and two monoclonal antibodies for the test of sars sandwich assay. Could you give me some suggestions ?Thank you!

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Answer

Thank you for your enquiry. I am sorry to confirm we do not have any SARS antibodies that are tested in sandwich ELISA (sELISA). SARS E2 antibody [4A6C9] (ab31951) and SARS Nucleoprotein antibody [BDI186] (ab28169) are tested in indirect ELISA only. I can suggest to consider trying one of them in an indirect ELISA method. Sandwich ELISA procedures can be difficult to optimise and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. I am sorry we have no suitably tested antibodies for sELISA on this occasion. I can suggest having a look on the Biocompare website. They have an excellent antibody search facility which includes a lot of suppliers. Regrettably, I am also not able to confirm that the ab28169 is raised against the SARS nucleoprotein, so will not detect the ab39839 E2 protein peptide. However, the ab39839 peptide has been specifically used as the immunogen for ab31951 SARS E2 antibody [4A6C9], so these can be used together in indirect ELISA. I hope this will be helpful. Should you have any further questions, please do not hesitate to contact us. Good luck with your research.

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