Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

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Overview

  • Product name

    Anti-SARS spike glycoprotein antibody [1A9]
    See all SARS spike glycoprotein primary antibodies

  • Description

    Mouse monoclonal [1A9] to SARS spike glycoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, Flow Cyt, IP, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV, SARS-CoV-2

  • Immunogen

    Tissue, cells or virus corresponding to Human coronavirus SARS spike glycoprotein aa 1029-1192.

  • Positive control

    • WB: infected caco-2 and SARS-CoV-2 transfected HEK-293T cell lysate. ICC/IF: SARS-CoV-2 transfected BHK-21, Vero E6, COS-7 cells. IP: SARS-CoV-2 transfected HEK-293T cells. Flow Cyt: Infected HEK-293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab273433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/2000.
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.
ELISA Use at an assay dependent concentration.

Assay dependent
Flow Cyt Use at an assay dependent concentration.

Assay dependent
IP Use at an assay dependent concentration.

Assay dependent
Sandwich ELISA Use at an assay dependent concentration.

Assay dependent

Target

  • Relevance

    A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.

  • Alternative names

    • E2 antibody
    • E2 glycoprotein antibody
    • Human coronavirus spike glycoprotein antibody
    • S antibody
    • S glycoprotein antibody
    • Spike glycoprotein antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Mock and SARS-CoV-2-transfected COS-7 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC/IF. Nuclei were counterstained with DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Methanol-fixed Vero E6 cells infected with SARS-CoV-2 (MOI of 1) stained for SARS-CoV-2 (green) using ab273433 at 1/2000 dilution in ICC/IF. Counterstained with DAPI (Blue).

  • Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Flow cytometric analysis of HEK-293T cells infected with recombinant vaccinia virus carrying the spike gene, using ab273433.

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extract
    Lane 2 : Transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody

    Predicted band size: 139 kDa

  • ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Sandwich ELISA detection of SARS spike glycoprotein using ab273433 as the capture antibody at 5 µg/mL. An alternative antibody was used as the detection antibody at 1 µg/mL followed by a Rabbit IgG antibody (HRP) at 1/10000 to detect the primary antibody.

  • Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    SARS-CoV-2 Spike was immunoprecipitated from 2μg of HEK-293T whole cell lysate with ab273433. Western blot was performed from the immunoprecipitate using ab273433. EasyBlot HRP-conjugated anti mouse IgG antibody.


    Lane 1: HEK-293T lysate 2 μg (Input).
    Lane 2: EasyBlot HRP-conjugated anti mouse IgG antibody.
    Lane 3: ab273433 IP in HEK-293T lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    4% paraformaldehyde-fixed BHK-21 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC/IF.

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lane 1 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lanes 2-3 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Mock
    Lane 2 : SARS-CoV infected Caco-2 whole cell extract
    Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extract

    Predicted band size: 139 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab273433? Please let us know so that we can cite the reference in this datasheet.

ab273433 has not yet been referenced specifically in any publications.

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