Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)
![Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-388068-Ant-sars-spike-glycoprotein-antibody-3a2-immunofluorescence-sars-infected-vero-e6-cells-coronavirus.jpg "Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)")
Key features and details
- Mouse monoclonal [3A2] to SARS spike glycoprotein - Coronavirus
- Suitable for: WB, ICC
- Isotype: IgG2b
Overview
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Product name
Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus
See all SARS spike glycoprotein primary antibodies -
Description
Mouse monoclonal [3A2] to SARS spike glycoprotein - Coronavirus -
Host species
Mouse -
Specificity
We have received mixed feedback regarding the specificity of this antibody against SARS-Cov2, please see the customer reviews section.
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Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: SARS-CoV
Does not react with: MERS-CoV -
Immunogen
Tissue, cells or virus corresponding to Human coronavirus SARS spike glycoprotein. (Formaldehyde inactivated SARS Coronavirus).
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Positive control
- ICC: SARS virus infected Vero E6 cells. WB: SARS virus infected cells.
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General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data Reviews: Customers have been successful and shared their AbReview
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3A2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 139 kDa.
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| ICC |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 139 kDa. |
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ICC
Use a concentration of 1 µg/ml. |
Target
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Relevance
A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. -
Alternative names
- E2 antibody
- E2 glycoprotein antibody
- Human coronavirus spike glycoprotein antibody
see all
Images
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Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)
Immunofluorescence analysis of SARS spike glycoprotein in the SARS virus infected cells with ab272420 antibody at 1/1000 dilution. (a) Uninfected Vero E6 cells. (b) SARS virus infected Vero E6 cells.
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![Western blot - Anti-SARS spike glycoprotein antibody [3A2] (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-388069-Ant-sars-spike-glycoprotein-antibody-3a2-western-blot-sars-infected-cells-coronavirus.jpg)
Western blot - Anti-SARS spike glycoprotein antibody [3A2] (ab272420)Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420) at 1/10000 dilution + Crude extract of the SARS virus infected cells
Predicted band size: 139 kDa -
![Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-392810-anti-sars-spike-glycoprotein-antibody-3a2-coronavirus-immunocytochemistry-vera-infected-sars-cov2.jpg)
Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)Immunofluorescence analysis of SARS spike glycoprotein in Vero cells infected with the SARS-CoV2 virus.
Left image: ab272420 (1 µg/mL)
Right image: Anti-SARS-CoV2 N protein monoclonal antibody
An Alexa 488-conjugated goat anti-mouse IgG (H+L) (1/2000) was used as the secondary antibody.
ab272420 does not cross-react with SARS-CoV2.
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![Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-392812-anti-sars-spike-glycoprotein-antibody-3a2-coronavirus-immunocytochemistry-vera-infected-mers-cov.jpg)
Immunocytochemistry - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)Immunofluorescence analysis of SARS spike glycoprotein in Vero cells infected with the MERS-CoV virus.
Left image: ab272420 (1 µg/mL)
Right image: Anti-MERS-CoV N protein monoclonal antibody
An Alexa 488-conjugated goat anti-mouse IgG (H+L) (1/2000) was used as the secondary antibody.
ab272420 does not cross-react with MERS-CoV.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab272420 has been referenced in 4 publications.
- Cantuti-Castelvetri L et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. Science 370:856-860 (2020). IHC-P ; Human . PubMed: 33082293
- Li SM et al. Reduced incorporation of SARS-CoV spike protein into viral particles due to amino acid substitutions within the receptor binding domain. Jpn J Infect Dis 61:123-7 (2008). WB, mIHC . PubMed: 18362400
- Yamashita M et al. Susceptibility of human and rat neural cell lines to infection by SARS-coronavirus. Biochem Biophys Res Commun 334:79-85 (2005). ICC/IF . PubMed: 15992768
- Yamate M et al. Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus. Microbes Infect 7:1530-40 (2005). Flow Cyt, ICC/IF . PubMed: 16269264