Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)
Key features and details
- Rabbit polyclonal to SARS spike glycoprotein - N-terminal
- Suitable for: WB, ELISA
- Isotype: IgG
Overview
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Product name
Anti-SARS spike glycoprotein antibody - N-terminal
See all SARS spike glycoprotein primary antibodies -
Description
Rabbit polyclonal to SARS spike glycoprotein - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Synthetic peptide corresponding to SARS spike glycoprotein (N terminal).
Database link: P59594 -
Positive control
- WB: SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 139 kDa.
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| ELISA |
Use at an assay dependent concentration.
ab252691 will detect 10 ng of free peptide at 1 µg/mL. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 139 kDa. |
|
ELISA
Use at an assay dependent concentration. ab252691 will detect 10 ng of free peptide at 1 µg/mL. |
Target
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Relevance
A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. -
Alternative names
- E2 antibody
- E2 glycoprotein antibody
- Human coronavirus spike glycoprotein antibody
see all
Images
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Western blot - Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)All lanes : Anti-SARS spike glycoprotein antibody - N-terminal (ab252691) at 1 µg/ml
Lane 1 : SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lane 2 : SARS-Cov2 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lane 3 : MERS Spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Green - ab252691 observed at 200kDa.
ab252691 was shown to react with SARS spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab252691 overnight at 4°C at 1 µg/ml. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) secondary antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab252691 has not yet been referenced specifically in any publications.
