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  1. Link

    sars-spike-glycoprotein-antibody-n-terminal-ab252691.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
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Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)

  • Datasheet
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Western blot - Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)

    Key features and details

    • Rabbit polyclonal to SARS spike glycoprotein - N-terminal
    • Suitable for: WB, ELISA
    • Isotype: IgG

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    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Primary
    Anti-SARS spike glycoprotein antibody - C-terminal (ab252690)
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    Recombinant SARS Nucleocapsid Protein (Coronavirus) (ab49042)

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    Overview

    • Product name

      Anti-SARS spike glycoprotein antibody - N-terminal
      See all SARS spike glycoprotein primary antibodies
    • Description

      Rabbit polyclonal to SARS spike glycoprotein - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WB, ELISAmore details
    • Immunogen

      Synthetic peptide corresponding to SARS spike glycoprotein (N terminal).
      Database link: P59594

    • Positive control

      • WB: SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • Other viruses

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab252691 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 139 kDa.
    ELISA
    Use at an assay dependent concentration.

    ab252691 will detect 10 ng of free peptide at 1 µg/mL.

    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 139 kDa.
    ELISA
    Use at an assay dependent concentration.

    ab252691 will detect 10 ng of free peptide at 1 µg/mL.

    Target

    • Relevance

      A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
    • Alternative names

      • E2 antibody
      • E2 glycoprotein antibody
      • Human coronavirus spike glycoprotein antibody
      • S antibody
      • S glycoprotein antibody
      • Spike glycoprotein antibody
      see all

    Images

    • Western blot - Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)
      Western blot - Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)
      All lanes : Anti-SARS spike glycoprotein antibody - N-terminal (ab252691) at 1 µg/ml

      Lane 1 : SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
      Lane 2 : SARS-Cov2 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
      Lane 3 : MERS Spike protein (DDDDK-tagged) transfected HEK-293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 139 kDa
      Observed band size: 200 kDa why is the actual band size different from the predicted?



      Lanes 1 - 3: Green - ab252691 observed at 200kDa.

      ab252691 was shown to react with SARS spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab252691 overnight at 4°C at 1 µg/ml. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) secondary antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab252691? Please let us know so that we can cite the reference in this datasheet.

    ab252691 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry abreview for Anti-SARS spike glycoprotein antibody - N-terminal

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (U2OS cells (Human Bone Osteosarcoma Epithelial Cel)
    Permeabilization
    Yes - 0.5% triton-X100
    Specification
    U2OS cells (Human Bone Osteosarcoma Epithelial Cel
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Lawrence Welch

    Verified customer

    Submitted Jun 16 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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