• Product name
  • Description
    Goat polyclonal to SART1
  • Host species
  • Specificity
    ab22820 is predicted to recognise both the human SART1(259) and SART1(800) proteins due to sequence homology, however we have only been able to detect the larger form by WB.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 2-14 of Human SART1.

  • Positive control
    • HeLa cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab22820 has been purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab22820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/64000.
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 90 kDa.


  • Relevance
    SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
  • Cellular localization
  • Database links
  • Alternative names
    • Ara 1 antibody
    • ARA1 antibody
    • HOMS 1 antibody
    • HOMS1 antibody
    • hSART 1 antibody
    • hSART1 antibody
    • hSnu 66 antibody
    • hSnu66 antibody
    • IgE autoantigen antibody
    • MGC2038 antibody
    • SART 1 antibody
    • SART1 259 antibody
    • SART1(259) protein antibody
    • SART1(800) protein antibody
    • SART1259 antibody
    • Snu 66 antibody
    • Snu66 antibody
    • Squamous cell carcinoma antigen recognised by T cells antibody
    • Squamous cell carcinoma antigen recognized by T cells 1 antibody
    • U4/U6.U5 tri snRNP associated 110 kDa protein antibody
    • U4/U6.U5 tri snRNP associated protein 1 antibody
    see all


  • Anti-SART1 antibody (ab22820) at 0.03 µg/ml + Hela lysate (35µg protein in RIPA buffer)

    Predicted band size: 90 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

    Primary incubation was 1 hour. Detected by chemiluminescence.


ab22820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your inquiry and I apologize for the delay while I was in touch with the testing lab.

As it turns out, this antibody is only "predicted" to recognize both human SART1(800) and SART1(259) proteinsbased on sequence homology, however only the larger form has been detected in their studies. I understand that the wording on the datasheet was misleading, and I have since updated it to state this fact (it will take a few days for our system to recache for it to display).

I apologize for the confusion. I would be happy to replace or refund this purchase. We do have 2 other anti-SART1 antibodies ab88583 and ab95957, but unfortunately I do not see any testing that shows the detection of a 43 kDa band.

Please let me know how you would like to proceed. I look forward to your reply.

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