Anti-SART1 antibody (ab88583)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-SART1 antibody
See all SART1 primary antibodies -
DescriptionMouse polyclonal to SART1
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
full length human SART1, amino acids 1-800 (NP_005137.1).
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Positive control
- SART1 transfected 293T cell lysate, Jurkat cell lysate, HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab88583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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RelevanceSART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 9092 Human
- Omim: 605941 Human
- SwissProt: O43290 Human
- Unigene: 502883 Human
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Alternative names
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
see all
Images
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Western blot - Anti-SART1 antibody (ab88583)Anti-SART1 antibody (ab88583) at 1 µg/ml + Jurkat cell lysate at 50 µg
Secondary
Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 110 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-SART1 antibody (ab88583)All lanes : Anti-SART1 antibody (ab88583) at 1 µg/ml
Lane 1 : SART1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution
Predicted band size: 90 kDa
Observed band size: 130 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (ab88583)ab88583 at 10µg/ml staining SART1 in HeLa cells by Immunofluorescence.
References
This product has been referenced in:
- Taylor SE et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol 6:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27655733) »
- Laetsch TW et al. Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma. Cell Death Dis 5:e1072 (2014). WB . Read more (PubMed: 24556687) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"