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    sart1-antibody-ab88583.pdf

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Anti-SART1 antibody (ab88583)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-SART1 antibody (ab88583)
  • Western blot - Anti-SART1 antibody (ab88583)
  • Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (ab88583)

Key features and details

  • Mouse polyclonal to SART1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-SART1 antibody
    See all SART1 primary antibodies
  • Description

    Mouse polyclonal to SART1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    full length human SART1, amino acids 1-800 (NP_005137.1).

  • Positive control

    • SART1 transfected 293T cell lysate, Jurkat cell lysate, HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Cell cycle
    • Cell differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Relevance

    SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 9092 Human
    • Omim: 605941 Human
    • SwissProt: O43290 Human
    • Unigene: 502883 Human
    • Alternative names

      • Ara 1 antibody
      • ARA1 antibody
      • HOMS 1 antibody
      • HOMS1 antibody
      • hSART 1 antibody
      • hSART1 antibody
      • hSnu 66 antibody
      • hSnu66 antibody
      • IgE autoantigen antibody
      • MGC2038 antibody
      • SART 1 antibody
      • SART1 259 antibody
      • SART1(259) protein antibody
      • SART1(800) protein antibody
      • SART1259 antibody
      • Snu 66 antibody
      • Snu66 antibody
      • Squamous cell carcinoma antigen recognised by T cells antibody
      • Squamous cell carcinoma antigen recognized by T cells 1 antibody
      • U4/U6.U5 tri snRNP associated 110 kDa protein antibody
      • U4/U6.U5 tri snRNP associated protein 1 antibody
      see all

    Images

    • Western blot - Anti-SART1 antibody (ab88583)
      Western blot - Anti-SART1 antibody (ab88583)
      Anti-SART1 antibody (ab88583) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Secondary
      Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution

      Predicted band size: 90 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-SART1 antibody (ab88583)
      Western blot - Anti-SART1 antibody (ab88583)
      All lanes : Anti-SART1 antibody (ab88583) at 1 µg/ml

      Lane 1 : SART1 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

      Predicted band size: 90 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (ab88583)
      Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (ab88583)
      ab88583 at 10µg/ml staining SART1 in HeLa cells by Immunofluorescence.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab88583? Please let us know so that we can cite the reference in this datasheet.

    ab88583 has been referenced in 3 publications.

    • Taylor SE  et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol 6:N/A (2016). ICC/IF ; Human . PubMed: 27655733
    • Laetsch TW  et al. Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma. Cell Death Dis 5:e1072 (2014). WB . PubMed: 24556687
    • Allen WL  et al. A systems biology approach identifies SART1 as a novel determinant of both 5-fluorouracil and SN38 drug resistance in colorectal cancer. Mol Cancer Ther 11:119-31 (2012). WB ; Human . PubMed: 22027693

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