Anti-SART1 antibody (ab88583)
Key features and details
- Mouse polyclonal to SART1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SART1 antibody
See all SART1 primary antibodies -
Description
Mouse polyclonal to SART1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
full length human SART1, amino acids 1-800 (NP_005137.1).
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Positive control
- SART1 transfected 293T cell lysate, Jurkat cell lysate, HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
|
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Relevance
SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9092 Human
- Omim: 605941 Human
- SwissProt: O43290 Human
- Unigene: 502883 Human
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Alternative names
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
see all
Images
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Western blot - Anti-SART1 antibody (ab88583)Anti-SART1 antibody (ab88583) at 1 µg/ml + Jurkat cell lysate at 50 µg
Secondary
Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Western blot - Anti-SART1 antibody (ab88583)All lanes : Anti-SART1 antibody (ab88583) at 1 µg/ml
Lane 1 : SART1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution
Predicted band size: 90 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (ab88583)ab88583 at 10µg/ml staining SART1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab88583 has been referenced in 3 publications.
- Taylor SE et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol 6:N/A (2016). ICC/IF ; Human . PubMed: 27655733
- Laetsch TW et al. Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma. Cell Death Dis 5:e1072 (2014). WB . PubMed: 24556687
- Allen WL et al. A systems biology approach identifies SART1 as a novel determinant of both 5-fluorouracil and SN38 drug resistance in colorectal cancer. Mol Cancer Ther 11:119-31 (2012). WB ; Human . PubMed: 22027693