Anti-SART1 antibody [EPR14563(B)] (ab181867)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-SART1 antibody [EPR14563(B)]
See all SART1 primary antibodies -
DescriptionRabbit monoclonal [EPR14563(B)] to SART1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human SART1 aa 650-750. The exact sequence is proprietary.
Database link: O43290 -
Positive control
- HeLa, Jurkat and MOLT4 cell lysates; HeLa and Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR14563(B)
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IsotypeIgG
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Research areas
Associated products
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Conjugation kits
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 90 kDa). | |
| ICC/IF | 1/250. | |
| Flow Cyt | 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceSART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 9092 Human
- Entrez Gene: 20227 Mouse
- Entrez Gene: 29678 Rat
- Omim: 605941 Human
- SwissProt: O43290 Human
- SwissProt: Q9Z315 Mouse
- SwissProt: Q5XIW8 Rat
- Unigene: 502883 Human
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Alternative names
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
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Images
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![Western blot - Anti-SART1 antibody [EPR14563(B)] (ab181867)](https://www.abcam.com/ps/products/181/ab181867/Images/ab181867-215322-ab181867.jpg)
Western blot - Anti-SART1 antibody [EPR14563(B)] (ab181867)All lanes : Anti-SART1 antibody [EPR14563(B)] (ab181867) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 90 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (ab181867)](https://www.abcam.com/ps/products/181/ab181867/Images/ab181867-215323-ab181867b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (ab181867)Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling SART1 with ab181867 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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![Flow Cytometry - Anti-SART1 antibody [EPR14563(B)] (ab181867)](https://www.abcam.com/ps/products/181/ab181867/Images/ab181867-215324-ab181867c.jpg)
Flow Cytometry - Anti-SART1 antibody [EPR14563(B)] (ab181867)Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Protocols
References
ab181867 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"