RecombinantRabMAb

Recombinant Anti-SART1 antibody [EPR14563(B)] (ab181867)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14563(B)] to SART1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SART1 antibody [EPR14563(B)]
    See all SART1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14563(B)] to SART1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SART1 aa 650-750. The exact sequence is proprietary.
    Database link: O43290

  • Positive control

    • HeLa, Jurkat and MOLT4 cell lysates; HeLa and Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14563(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 90 kDa).
ICC/IF 1/250.
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.

  • Cellular localization

    Nuclear

  • Database links

    see all

  • Alternative names

    • Ara 1 antibody
    • ARA1 antibody
    • HOMS 1 antibody
    • HOMS1 antibody
    • hSART 1 antibody
    • hSART1 antibody
    • hSnu 66 antibody
    • hSnu66 antibody
    • IgE autoantigen antibody
    • MGC2038 antibody
    • SART 1 antibody
    • SART1 259 antibody
    • SART1(259) protein antibody
    • SART1(800) protein antibody
    • SART1259 antibody
    • Snu 66 antibody
    • Snu66 antibody
    • Squamous cell carcinoma antigen recognised by T cells antibody
    • Squamous cell carcinoma antigen recognized by T cells 1 antibody
    • U4/U6.U5 tri snRNP associated 110 kDa protein antibody
    • U4/U6.U5 tri snRNP associated protein 1 antibody
    see all

Images

  • Western blot - Anti-SART1 antibody [EPR14563(B)] (ab181867)
    Western blot - Anti-SART1 antibody [EPR14563(B)] (ab181867)
    All lanes : Anti-SART1 antibody [EPR14563(B)] (ab181867) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MOLT4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (ab181867)
    Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (ab181867)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling SART1 with ab181867 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry - Anti-SART1 antibody [EPR14563(B)] (ab181867)
    Flow Cytometry - Anti-SART1 antibody [EPR14563(B)] (ab181867)

    Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-SART1 antibody [EPR14563(B)] (ab181867)
    Anti-SART1 antibody [EPR14563(B)] (ab181867)

References (0)

Publishing research using ab181867? Please let us know so that we can cite the reference in this datasheet.

ab181867 has not yet been referenced specifically in any publications.

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