Recombinant Anti-SART1 antibody [EPR14563(B)] (ab181867)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14563(B)] to SART1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SART1 antibody [EPR14563(B)]
See all SART1 primary antibodies -
Description
Rabbit monoclonal [EPR14563(B)] to SART1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat and MOLT4 cell lysates; HeLa and Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14563(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 90 kDa).
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ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 90 kDa). |
ICC/IF
1/250. |
Target
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Function
Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the SNU66/SART1 family. -
Post-translational
modificationsSumoylated with SUMO2. -
Cellular localization
Nucleus. Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. - Information by UniProt
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Database links
- Entrez Gene: 9092 Human
- Entrez Gene: 20227 Mouse
- Entrez Gene: 29678 Rat
- Omim: 605941 Human
- SwissProt: O43290 Human
- SwissProt: Q9Z315 Mouse
- SwissProt: Q5XIW8 Rat
- Unigene: 502883 Human
see all -
Alternative names
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
see all
Images
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All lanes : Anti-SART1 antibody [EPR14563(B)] (ab181867) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 90 kDa -
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling SART1 with ab181867 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181867 has not yet been referenced specifically in any publications.