Overview

  • Product name

  • Description

    Rabbit polyclonal to SART3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide within Human SART3 aa 600-650. The exact sequence is proprietary.
    Sequence:

    Q RKRARAEKKA LKKKKKIRGP EKRGADEDDE KEWGDDEEEQ PSKRRRVENS


    Database link: Q15020

  • Positive control

    • HeLa whole cell lysate (ab150035); Human ovarian and colon carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab176822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
ICC/IF 1/250 - 1/1000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
  • Sequence similarities

    Contains 8 HAT repeats.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
  • Information by UniProt
  • Database links

  • Alternative names

    • DSAP1 antibody
    • hSART 3 antibody
    • hSART-3 antibody
    • P100 antibody
    • P110 antibody
    • p110(nrb) antibody
    • RP11 13G14 antibody
    • SART 3 antibody
    • SART-3 antibody
    • SART3 antibody
    • SART3_HUMAN antibody
    • Squamous cell carcinoma antigen recognized by T cells 3 antibody
    • Squamous cell carcinoma antigen recognized by T-cells 3 antibody
    • Tat interacting protein of 110 kDa antibody
    • Tat-interacting protein of 110 kDa antibody
    • Tip110 antibody
    see all

Images

  • All lanes : Anti-SART3 antibody (ab176822) at 0.02 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 110 kDa


    Exposure time: 3 seconds
  • Detection of SART3  by Western Blot of Immunoprecipitate.
    abX176822 at 0.04µg/ml labeling SART3  in HeLa whole cell lysate immunoprecipitated using ab176822 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure times of 3
    seconds.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling SART3 with ab176822 at 1 µg/ml.

    Detection: DAB staining.

  • Immunohistofluorescent analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SART3 with ab176822 at 0.5 µg/ml.
    Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100.

References

ab176822 has not yet been referenced specifically in any publications.

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