Key features and details
- Rabbit polyclonal to SART3
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SART3 antibody
See all SART3 primary antibodies
DescriptionRabbit polyclonal to SART3
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
- HeLa whole cell lysate (ab150035); Human ovarian and colon carcinoma tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176822 was affinity purified using an epitope specific to SART3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 110 kDa.|
|ICC/IF||1/250 - 1/1000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRegulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
Sequence similaritiesContains 8 HAT repeats.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
- Information by UniProt
- DSAP1 antibody
- hSART 3 antibody
- hSART-3 antibody
All lanes : Anti-SART3 antibody (ab176822) at 0.02 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 3 seconds
Detection of SART3 by Western Blot of Immunoprecipitate.
abX176822 at 0.04µg/ml labeling SART3 in HeLa whole cell lysate immunoprecipitated using ab176822 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure times of 3
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling SART3 with ab176822 at 1 µg/ml.
Detection: DAB staining.
Immunohistofluorescent analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SART3 with ab176822 at 0.5 µg/ml.
Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100.
ab176822 has not yet been referenced specifically in any publications.