The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 110 kDa. (predicted molecular weight: 110 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
Involvement in disease
Defects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Approx 150kDa band observed in lysates of Human Tcell line Jurkat and Human Burkitt's Lymphoma cell line DAUDI (calculated MW of 111kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature (Yang et al, Cancer Res. 1999 Aug 15;59(16):4056-63; PMID: 10463607).
Long L et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem289:8916-30 (2014).
Read more (PubMed: 24526689) »