Key features and details
- Rabbit polyclonal to SASH3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SASH3 antibody
DescriptionRabbit polyclonal to SASH3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- IHC-P: Human lymph node, duodenum, testis and tonsil tissue. WB: HEK-293T overexpressing human SASH3 with a C-terminal myc-DDK tag, whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab251656 in the following tested applications.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionMay function as a signaling adapter protein in lymphocytes.
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.
- Information by UniProt
- 1200013B08Rik antibody
- 753P9 antibody
- AW413946 antibody
All lanes : Anti-SASH3 antibody (ab251656) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T overexpressing human SASH3 with a C-terminal myc-DDK tag, whole cell lysate.
Predicted band size: 42 kDa
Paraffin-embedded human skeletal muscle tissue stained for SASH3 using ab251656 at 1/50 dilution in immunohistochemical analysis. Low expression, as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab251656 has not yet been referenced specifically in any publications.