Key features and details
- Rabbit polyclonal to SASS6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SASS6 antibody
See all SASS6 primary antibodies
DescriptionRabbit polyclonal to SASS6
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
Synthetic peptide corresponding to a region between residues 607 and 657 of Human SASS6 (NP_919268.1).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceSASS6 (Spindle assembly abnormal protein 6 homolog) is required for centrosome duplication. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. Overexpression results in excess foci bearing centriolar markers.
Cellular localizationCytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; centrosome; centriole. Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis.
- 2810453L12Rik antibody
- FLJ22097 antibody
- HsSAS 6 antibody
All lanes : Anti-SASS6 antibody (ab85993) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 3 minutes
Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab85993 has not yet been referenced specifically in any publications.