SAT-alpha positive control ChIP primer pair (ab269263)
Key features and details
- SAT-alpha positive control ChIP primer pair
- Suitable for: ChIP
Overview
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Product name
SAT-alpha positive control ChIP primer pair -
Tested applications
Suitable for: ChIPmore details -
General notes
Positive control ChIP-qPCR 5' and 3' primers for SAT-alpha. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.
Suitable positive control for:
- Histone H3 tri methyl K9
- Histone H3 phospho S28500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
Properties
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Form
Lyophilized -
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading... -
Clonality
Monoclonal
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
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| Notes |
|---|
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ChIP
Use at an assay dependent concentration. |
Images
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ChIP - SAT-alpha positive control ChIP primer pair (ab269263)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176916 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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ChIP - SAT-alpha positive control ChIP primer pair (ab269263)Chromatin was prepared from HeLa cells according to theAbcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab32388 (red), and 20 µl of Protein A/G sepharose beads. Rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab269263 has not yet been referenced specifically in any publications.
