Product nameAnti-SATB1 antibody
See all SATB1 primary antibodies
DescriptionRabbit polyclonal to SATB1
SpecificityThis antibody is specific for SATB1
Tested applicationsSuitable for: ELISA, IP, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat
- Jurkat cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab49061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 86 kDa).Can be blocked with Human SATB1 peptide (ab111669). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionCrucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
Tissue specificityExpressed predominantly in thymus.
Sequence similaritiesBelongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.
modificationsSumoylated. Sumoylation promotes cleavage by caspases.
Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
Cellular localizationNucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- DNA binding protein SATB1 antibody
- DNA-binding protein SATB1 antibody
- SATB homeobox 1 antibody
Lane 1 : Anti-SATB1 antibody (ab49061) at 1.25 µg/ml
Lane 2 : Anti-SATB1 antibody (ab49061) at 2.5 µg/ml
Lane 3 : Anti-SATB1 antibody (ab49061) at 5 µg/ml
All lanes : Cell lysate prepared from human Jurkat cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 86 kDa
ab49061 staining SATB1 in HEK 293T cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with formaldehyde, permeabilized using 0.1 % Triton X-100 for 5 minutes, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab49061 at a 1/100 dilution for 3 hours at 25°C. The secondary used was an Oregon Green 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
Estrogen Related Receptor gamma was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor gamma and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49061.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 100KDa: Estrogen Related Receptor gamma
This product has been referenced in:
- Gao XY et al. Effect of baicalein on the expression of SATB1 in human breast cancer cells. Exp Ther Med 9:1665-1669 (2015). Read more (PubMed: 26136875) »
- Zhang S et al. Expression and significance of SATB1 in the development of breast cancer. Genet Mol Res 14:3309-17 (2015). Read more (PubMed: 25966097) »