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    satb1-antibody-ab70004.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-SATB1 antibody (ab70004)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (13)

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Western blot - Anti-SATB1 antibody (ab70004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody (ab70004)
  • Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody (ab70004)

Key features and details

  • Rabbit polyclonal to SATB1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SATB1 antibody
    See all SATB1 primary antibodies
  • Description

    Rabbit polyclonal to SATB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    18 amino acid peptide near the amino terminus of the human SATB1 (GenBank accession no. Q01826).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • SK-N-BE(2) nuclear extract lysate (ab14894)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100, 110 kDa (predicted molecular weight: 85 kDa).
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2.5 µg/ml.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100, 110 kDa (predicted molecular weight: 85 kDa).
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2.5 µg/ml.

Target

  • Function

    Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
  • Tissue specificity

    Expressed predominantly in thymus.
  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated. Sumoylation promotes cleavage by caspases.
    Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
    Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
  • Cellular localization

    Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
  • Target information above from: UniProt accession Q01826 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6304 Human
    • Omim: 602075 Human
    • SwissProt: Q01826 Human
    • Unigene: 517717 Human
    • Form

      There are 2 isoforms produced by alternative splicing.
    • Alternative names

      • DNA binding protein SATB1 antibody
      • DNA-binding protein SATB1 antibody
      • SATB homeobox 1 antibody
      • SATB1 antibody
      • SATB1_HUMAN antibody
      • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
      • Special AT rich sequence binding protein 1 antibody
      • Special AT-rich sequence-binding protein 1 antibody
      see all

    Images

    • Western blot - Anti-SATB1 antibody (ab70004)
      Western blot - Anti-SATB1 antibody (ab70004)
      Lane 1 : Anti-SATB1 antibody (ab70004) at 1 µg/ml
      Lane 2 : Anti-SATB1 antibody (ab70004) at 2 µg/ml

      All lanes : SK-N-SH cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 85 kDa
      Additional bands at: 100 kDa (possible isoform), 110 kDa (possible isoform)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody (ab70004)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody (ab70004)

      ab70004 at 2.5µg/ml staining SATB1 in human brain tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

    • Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody (ab70004)
      Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody (ab70004)
      Immunofluorescence of SATB1 in Human Brain cells using ab70004 at 20 ug/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (13)

    Publishing research using ab70004? Please let us know so that we can cite the reference in this datasheet.

    ab70004 has been referenced in 13 publications.

    • Seo W  et al. Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity. Nat Commun 11:1562 (2020). PubMed: 32218434
    • Yin CY  et al. miR-7-5p inhibits cell migration and invasion in glioblastoma through targeting SATB1. Oncol Lett 17:1819-1825 (2019). PubMed: 30675243
    • Yasuda K  et al. Satb1 regulates the effector program of encephalitogenic tissue Th17 cells in chronic inflammation. Nat Commun 10:549 (2019). PubMed: 30710091
    • Salib M  et al. GABAergic Medial Septal Neurons with Low-Rhythmic Firing Innervating the Dentate Gyrus and Hippocampal Area CA3. J Neurosci 39:4527-4549 (2019). PubMed: 30926750
    • Viney TJ  et al. Shared rhythmic subcortical GABAergic input to the entorhinal cortex and presubiculum. Elife 7:N/A (2018). PubMed: 29620525
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Should the product be stored at +4C for long term storage?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 29 2013

    Answer

    Thank you for your enquiry.

    This product can be stored at 4˚C for three months and -20˚C for long-term, stable for up to one year. Please avoid freeze/thaw cycles.

    Please let us know if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 29 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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