Product nameAnti-SATB1 antibody
See all SATB1 primary antibodies
DescriptionRabbit polyclonal to SATB1
Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
18 amino acid peptide near the amino terminus of the human SATB1 (GenBank accession no. Q01826).
- SK-N-SH cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 23686316|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100, 110 kDa (predicted molecular weight: 85 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionCrucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
Tissue specificityExpressed predominantly in thymus.
Sequence similaritiesBelongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.
modificationsSumoylated. Sumoylation promotes cleavage by caspases.
Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
Cellular localizationNucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- DNA binding protein SATB1 antibody
- DNA-binding protein SATB1 antibody
- SATB homeobox 1 antibody
Lane 1 : Anti-SATB1 antibody (ab70004) at 1 µg/ml
Lane 2 : Anti-SATB1 antibody (ab70004) at 2 µg/ml
All lanes : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 85 kDa
Additional bands at: 100 kDa (possible isoform), 110 kDa (possible isoform)
ab70004 at 2.5µg/ml staining SATB1 in human brain tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunofluorescence of SATB1 in Human Brain cells using ab70004 at 20 ug/ml.
This product has been referenced in:
- Viney TJ et al. Shared rhythmic subcortical GABAergic input to the entorhinal cortex and presubiculum. Elife 7:N/A (2018). Read more (PubMed: 29620525) »
- Kitagawa Y et al. Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment. Nat Immunol 18:173-183 (2017). Read more (PubMed: 27992401) »