Overview

  • Product name

    Anti-SATB1 antibody - C-terminal
    See all SATB1 primary antibodies
  • Description

    Rabbit polyclonal to SATB1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SATB1 (C terminal). The exact sequence is proprietary.
    Database link: Q01826

  • Positive control

    • WB: Jurkat and THP-1 whole cell lysates; Mouse thymus tissue extract. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue. ChIP: Jurkat chromatin extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 86 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ChIP Use at an assay dependent concentration.

Target

  • Function

    Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
  • Tissue specificity

    Expressed predominantly in thymus.
  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated. Sumoylation promotes cleavage by caspases.
    Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
    Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
  • Cellular localization

    Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • DNA binding protein SATB1 antibody
    • DNA-binding protein SATB1 antibody
    • SATB homeobox 1 antibody
    • SATB1 antibody
    • SATB1_HUMAN antibody
    • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
    • Special AT rich sequence binding protein 1 antibody
    • Special AT-rich sequence-binding protein 1 antibody
    see all

Images

  • Anti-SATB1 antibody - C-terminal (ab228772) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

    Predicted band size: 86 kDa



    7.5% SDS-PAGE gel.

  • SATB1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab228772. Western blot was performed from the immunoprecipitate using ab228772 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: 2 μg of preimmune rabbit IgG instead of ab228772 in HEK-293T whole cell lysate.
    Lane 2: ab228772 IP in HEK-293T whole cell lysate.

  • Paraffin-embedded human colon carcinoma tissue stained for SATB1 using ab228772 at 1/250 dilution in immunohistochemical analysis.

  • Cross-linked ChIP was performed with Jurkat (human T cell leukemia cell line from peripheral blood) chromatin extract and 5 μg of either control rabbit IgG or ab228772. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.

  • Anti-SATB1 antibody - C-terminal (ab228772) at 1/500 dilution + THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg

    Predicted band size: 86 kDa



    7.5% SDS-PAGE gel.

  • Anti-SATB1 antibody - C-terminal (ab228772) at 1/5000 dilution + Mouse thymus tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 86 kDa



    7.5% SDS-PAGE gel.

References

This product has been referenced in:

  • Sun L  et al. SATB1 3'-UTR and lncRNA-UCA1 competitively bind to miR-495-3p and together regulate the proliferation and invasion of gastric cancer. J Cell Biochem 120:6671-6682 (2019). Read more (PubMed: 30368875) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228772.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up