Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

Overview

  • Product name

    Anti-SATB1 antibody [EPR3951] - BSA and Azide free
    See all SATB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3951] to SATB1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, IHC-Fr, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SATB1. The exact sequence is proprietary.

  • General notes

    Ab239944 is the carrier-free version of ab109122. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239944 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22123820
WB Use at an assay dependent concentration. Predicted molecular weight: 86 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
    • Tissue specificity

      Expressed predominantly in thymus.
    • Sequence similarities

      Belongs to the CUT homeobox family.
      Contains 2 CUT DNA-binding domains.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications

      Sumoylated. Sumoylation promotes cleavage by caspases.
      Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
      Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
    • Cellular localization

      Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
    • Information by UniProt
    • Database links

    • Form

      There are 2 isoforms produced by alternative splicing.
    • Alternative names

      • DNA binding protein SATB1 antibody
      • DNA-binding protein SATB1 antibody
      • SATB homeobox 1 antibody
      • SATB1 antibody
      • SATB1_HUMAN antibody
      • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
      • Special AT rich sequence binding protein 1 antibody
      • Special AT-rich sequence-binding protein 1 antibody
      see all

    Images

    • Ab109122 staining SATB1 in THP-1 (Human monocytic leukemia monocyte) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized in 0.1% TritonX-100. Samples were incubated with primary antibody 1:1000 dilution (2.1 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Alexa Fluor® 594 Anti-alpha Tubulin [DM1A] – Microtubule marker, ab195889 was used as a counterstain antibody (1:200, 2.5 μg/ml). DAPI was used as a counterstain antibody. Confocal image showing nuclear staining on THP-1 cell line. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    • Ab109122 staining SATB1 in Jurkat (Human T cell leukemia T lymphocyte) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized in 0.1% TritonX-100. Samples were incubated with primary antibody 1:1000 dilution (2.1 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Alexa Fluor® 594 Anti-alpha Tubulin [DM1A] – Microtubule marker, ab195889 was used as a counterstain antibody (1:200, 2.5 μg/ml). DAPI was used as a counterstain antibody. Confocal image showing nuclear staining on Jurkat cell line.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    • ab109122 staining SATB1 in mouse skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde, blocked with 10% goat serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 12 hours. A FITC-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    • ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human breast carcinoma, by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    • ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human ovarian adenocarcinoma tissue, by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    • ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    References

    ab239944 has not yet been referenced specifically in any publications.

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