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I don't think the problem can be degradation, since two of the bands are higher than the expected 85kDa band.
Asked on Oct 27 2011
Looking the images you sent, the blot on the bottom right, shows a band at approximately 75kDa and a band below 100kDa, could this be the 85kDa band? There is also a band just above that. Does SATB2 undergo any post-translational modifications that could account for the higher band? As I mentioned previously, this antibody is covered under our Abpromise. Therefore as this antibody is not working as guaranteed, then I would be happy to send you another SABT2 antibody as a replacement.
Answered on Oct 27 2011