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I dread doing a 4th western with this antibody- if you can send a new vial to my address here that would be great. It was originally purchased by my collaborator at AFFRI (Dr. Alexandra Miller) and mailed to her. Is there any way you can mail it to me directly? If not, she'll be able to re-ship it to me, but of course that is extra hassle for her and more time wasted for me. If possible the address here is: I'm going to try another whole-cell lysate with one extra protease inhibitor and plan to repeat with fewer proteins as you suggested, but I'm just skeptical that it will work this time. Do you have any idea why the half of the membrane blocked with milk shows nothing at all? Thanks,
Asked on Nov 02 2011